摘要
目的 :研究子宫内膜异位症患者子宫在位内膜和异位内膜IL 18的表达 ,探讨IL 18在内异症发病机制中的意义。方法 :用免疫组化和半定量逆转录聚合酶链反应法检测对照组子宫内膜、子宫腺肌症患者子宫内膜和内异症患者的子宫在位内膜与异位内膜IL 18蛋白的表达位置及其mRNA表达水平。结果 :各组标本IL 18蛋白和mRNA的表达均阳性 ,内异症患者子宫在位内膜与异位内膜的IL 18mRNA表达水平分别为 1.0 5±0 .4 0、0 .77± 0 .39,均低于对照组子宫内膜 (1.6 5± 0 .6 4) ,差异有显著性 (P <0 .0 5 ) ;子宫腺肌症组IL 18mRNA表达水平为 1.6 3± 0 .6 0 ,与对照组子宫内膜IL 18mRNA表达水平差异无显著性。结论 :IL 18可能参与了子宫内膜异位症的发病 ,IL 18mRNA在内异症患者在位和异位内膜的低水平表达 ,可能是影响内异症形成和发展的重要因素之一。
Objective:To study the expression of IL-18 in eutopic and ectopic endometriun of endometriosis and the role on the pathogenesis of this disease.Methods:Endometrium specimens were obtained from 22 normal uteruses,26 with endometriosis and 21 with adenomyosis.Endometriosic tissues were obtained from 28 patients.Immunohistochemistry was employed to determine the location of IL-18 protein,and semiquantitative reverse transcriptase polymerase chain reaction,using β-actin as internal standard,was applied to determine the levels of IL-18 mRNA in those specimens.Results:IL-18 protein was found in all specimens and the expression of IL-18 mRNA was significantly lower in ectopic and eutopic endometrium from patients with endometriosis, compared with those from normal women and patients with adenomyosis (0.77±0.39, 1.05±0.40 and 1.65±0.64,1.63±0.60,respectively,P<0.05).In normal women,expression of IL-18 mRNA was higher in secretory endometrium than that in proliferative endometrium;whereas in endometriosic women,there was no difference.Conclusion:Women with endometriosis show down-regulation of IL-18 expression in ectopic and eutopic endometrium, and the abnormal expression may result in formation and continuing growth of ectopic locations.
出处
《现代妇产科进展》
CSCD
2004年第3期194-196,共3页
Progress in Obstetrics and Gynecology
