摘要
初步建立了兔肝素化血浆脂蛋白脂酶(LPL)和肝脂酶(HL)活性的测定方法,两种脂酶活性以水解三油酸甘油酯(TG)生成游离脂肪酸(FFA)的含量表示。测得雄性日本大耳白兔肝素化后15min血浆LPL、HL活性最大,批内差异分别为4.96%、3.26%;正常兔LPL、HL分别为12.15±4.07μmol·L^(-1)FFA/h·ml、17.11±6.80μmol·L^(-1)FFA/h·ml;实验兔喂饲胆固醇12wk形成动脉粥样硬化(AS)时LPL、HL活性分别为7.94±3.36μmol·L^(-1)FFA/h·ml、4.54±1.52μmol·L^(-1)FFA/h·ml,两者均较正常兔明显降低(P<0.005)。提示:在高胆固醇负荷时,LPL、NL活性降低可能促进了AS的形成。
A method for the selective measurement of post heparin plasma lipoprotein lipase (EC 3·1·1·34 LPL) and hepatic lipase (HL) in rabbit was described, In both assays, enzymatic activity was determined by measuring free fatty acid (FFA) liberated from triolen. The activities of both lipases were maximum at 15 min after heparinization and cofficient of variation within batch were 4.96% and 3.26% respectively. In normal rabbits, the activities of LPL and HL were 12.15±4.07 and 17.11±6.80 μmol·L^(-1)FFA/h·ml respectively. The activities of lipases were significantly lower than that of control group in rabbit with expermental atherosclerosis after fed cholestrol for 12 weeks. The above results suggested that the descended activities of LPL and HL may promote atherosclerosis in rabbit fed Cholesterol-enriched diet.
出处
《安徽医科大学学报》
CAS
1993年第2期84-87,共4页
Acta Universitatis Medicinalis Anhui
关键词
脂肪酶
动脉粥样硬化
脂蛋白
lipoprotein lipase
atherosclerosis
apolipoproteins
HDL
LDL
VLDL
rabbits
