摘要
目的:在兔肝VX-2移植瘤模型基础上,进行在体肝肿瘤介入性热化疗,观察介入性热增敏与肝组织及肿瘤组织血管渗透性的关系,探讨介入性热化疗的生物学机制。方法:建立可供实验研究的兔Vx-2移植性肝癌模型30只,随机等分为3组(非灌注组、普通灌注组及热灌注组),在X线监视下,经股动脉插管至肝动脉。进行灌注,灌注液为生理盐水(温度为60℃),液量30mL,15min缓慢推注。灌注结束前5min,经导管推注10mg/L伊文思蓝(EvansBlueEB),2mL/kg,非灌注组直接推注EB,灌注EB后置10min,取出肝脏用生理盐水灌注肝动脉冲洗血管中残留的EB。切取靠近肝门部的小块正常肝组织、瘤组织,称重后,放入1mL甲酰胺液中,置于50℃恒温水浴箱60h,提取液用722型光栅分光光度计测出A620nm,从标准曲线上测出相应的EB含量,以反映该组织毛细血管的渗透性。结果:肿瘤组织与肝组织的EB含量在3组中均有差别(P<0.05);正常灌注组与非灌注组肝组织的EB含量、肿瘤组织的EB含量无明显差别(P>0.05);热灌注组与非灌注组、正常灌注组肝组织的EB含量、肿瘤组织的EB含量有明显差别(P<0.05)。结论:介入热疗可以增加肝组织及肿瘤组织的血管渗透性。
AIM:Interventional chemothermotherapy in hepatocellular carcinoma was operated in vivo on the basis of liver VX 2 transplanted hepatocellular carcinoma model in rabbit.The relationships between interventional chemothermotherapy and liver tissue as well as the vascular penetrability were observed for the discussion of the biological mechanism of interventional chemothermotherapy. METHODS:Thirty models of VX 2 transplanted hepatocellular carcinoma in rabbits were established for research.Rabbits were randomly allocated into 3 groups(non perfusion group,normal temperature perfusion group and thermo perfusion group).Under the surveillance of X ray,30 mL of NS(60 ℃) were slowly injected into liver artery through the intubation from femoral artery within 15 minutes for perfusion.At 5 minutes before the completion of perfusion,10 mg/L of EB was injected through ductus with a dose of 2 mL/kg.EB was directly injected into non perfusion group.Liver was removed at 10 minutes after EB perfusion and the residual of EB in vessels was rinsed by NS.Small pieces of normal liver tissue and carcinoma tissue closed to hepatic portal were cut and put into 1 mL formamide solution after weighing.Specimens then were put into constant temperature water bath cabinet for 60 hours at 50 ℃.A620nm was assayed by 722 model grating spectrophotometryand the corresponding EB content was measured on standard curve to reflect the penetrability of capillary in this piece of tissue. RESULTS:There were differences of EB contents in liver and carcinoma tissues among three groups(P< 0.05).There were no significant differences of EB contents in liver and carcinoma tissue between non perfusion group and normal temperature perfusion group(P >0.05).There were significant differences of EB contents in liver and carcinoma tissue between thermo perfusion group and non perfusion group,and between thermo perfusion group and normal temperature perfusion group as well(P< 0.05). CONCLUSION:Interventional chemothermotherapy can increase vascular penetrability in liver and carcinoma tissues.
出处
《中国临床康复》
CSCD
2004年第14期2778-2779,共2页
Chinese Journal of Clinical Rehabilitation
基金
全军"十五"医学青年基金(01Q124)
第四军医大学科技创新工程基金(CX99A016)
陕西省社发攻关课题(2003K10G64)~~
