摘要
自从1958年Moore等人建立氨基酸测定方法以来,氨基酸分析方法发展迅速,日新月异。除采用阳离子交换柱,茚三酮柱后反应测定氨基酸方法外,70年代起,各种采用柱前衍生,反相色谱法分离测定氨基酸方法相继问世,为氨基酸分析开辟了新的领域。异硫氢酸苯酯(PITC)法作为其中一种,以其灵敏度高(可检测,1Pmal),分析速度快(水解液为10分钟。
This review considers the recent development and application of analytical procedures for phenylisothiocyanate-derivatized amino acids. The range of applications requiring the analysis of femtomole to micromole amounts in samples ranging in analytical biochemcal studies, through body fluid samples in clinical
chemistry, to feed stocks in agricultural and food chemistry.
