摘要
目的研究紫杉醇溶液外用对豚鼠耳部银屑病样病理改变的治疗作用。方法5%普萘洛尔软膏外涂豚鼠耳部皮肤,使其产生银屑病样病理改变,然后分别以200μg/mL和500μg/mL的紫杉醇溶液外涂造模部位进行治疗,光镜下观察局部皮肤病理变化。结果造模后的组织病理可见角化过度,角化不全,棘层肥厚,真皮毛细血管扩张,有较明显的单一核细胞及多形核白细胞浸润,Baker评分为6.37±0.99。外涂200μg/mL和500μg/mL紫杉醇溶液治疗后,造模处皮损的组织病理明显改善,Baker评分分别下降至3.13±1.13及2.13±0.81。治疗组与造模组及基质对照组比较,差异有显著性(P<0.05),未发现紫杉醇溶液外涂引起的局部毒副作用。结论紫杉醇溶液对普萘洛尔诱发的豚鼠耳部上皮增殖及炎症现象具有抑制作用。
Objective To observe the therapeutic effects of paclitaxel solution on psoriasis-like pathological changes of guinea pig.Methods5%propranolol ointment was applied on Guinea pigs' ears to produce psoriasis-like pathological changes,then solutions with a paclitaxel concentration of200μg/mL and500μg/mL were applied on the affected ears.The pathological manifestations were investigated under light microscope.Results There were hyperkeratosis,dyskeratosis,acanthosis,psoriasiform elongation of the rete ridges and significant infiltration of mononuclear cells and polymorphic nucleus leucocytes in dermis in the guinea pig models,with the Baker score reaching6.37±0.99averagely.After being treated with paclitaxel solutions of a concentration of200μg/mL and500μg/mL,the pathological manifestations were remarkably improved,with the Baker score reducing to3.13±1.13and2.13±0.81respectively.A significant differ-ence(P<0.05)of the improvement of Baker score was observed between Guinea pig models treated with low and high concentrations of paclitaxel,and between models and control animals.Toxic effects on ear sites due to local application of paclitaxel solutions were not observed.Conclusion Paclitaxel solutions might have an inhibitive effect on the proliferation and inflammation in epidermis of Guinea pigs' ears induced by propranolol ointment.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2004年第3期156-158,共3页
Chinese Journal of Dermatology
基金
浙江省卫生厅(编号:G29932)
浙江省教委资助课题(981058)
