摘要
我们通过培养来源于慢性胃炎患者胃液的染色曲霉,分离制备了包括杂色曲霉素(ST)、averufin(AV)和新产物5′-hydroxy-averantin(5′-HA)等代谢产物。并检测它们及其有关衍生物的致突变性。Ames试验表明:在0.1～100μg/皿剂量时,ST、O—甲基—ST、O—乙酰基—ST,不论有无S_θ存在,都显示阳性结果,而AV和5′-HA只是在S_θ存在时出现阳性;加S_θ时5′-HA和AV对TA_(θ8)和TA_(100)菌株的致突变性强度分别是AFB_1的0.02％和0.03％、0.04％,而ST则是13.75％和38.35％。在S_θ存在时ST、5′-HA、AV对CHL细胞染色体畸变阳性剂量分别是0.05、10.0、50.0μg/ml,不加S_θ,只有ST在5μg/ml时就出现阳性。染色体畸变类型,ST和AV以cte为主。而5′-HA以ctb为主。据此认为新产物5′-HA和AV的致突变性,远远弱于ST和AFB_1。
Aspergillus versicolor isolated from gastric juice of patients with chronic gastritis was incubated. Sterigmatocystin (ST), averufin (AV) and a new metabolite 5'-hydroxy-averantin (5'-HA) were purified after these cultured samples were extracted and separated. These three metabolites and ST related compounds were examined for their mutagenicities with Ames test and chromosomal aberration assay was adopted.In Ames test, Salmonella typhimuriumTA 98,TA100 were used, When the doses from 0.1 μg/plate to 100 μg/plate, ST, O-methyl-ST and O-acetyl-ST were positive no matter how S9 mix existed or not. But AV and 5'-HA only showed positive in the presence S8 mix.Comparing with AFB1 in the presence of S9 mix, the relative mutagenic activity(RMA%) to TA98 and TA100 of 5'-HA were 0.02, 0.03; AV were 0.02, 0.04; ST were 13.75, 38.35. CHL cells were used in the chromosomal aberration assay. In the presence of S9 mix, the positive dose of ST, 5'-HA and AV were 0.05 μg/ml, 10 μg/ml and 50 μg/ml, respectively.Only ST was positive at 5.0 μg/ml level without S9 mix. The main type of chromosomal aberration for ST and AV was cte and 5'-HA was ctb. These results suggested that the mutagenicities of AV and the new metabolite 5'-HA are weaker than that of ST and AFB1.
出处
《癌变·畸变·突变》
CAS
CSCD
1993年第1期13-19,共7页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
WHO奖学金资助
关键词
杂色曲霉
杂色曲霉素
致突变性
Aspergillus rersicolor
sterigmatocystin
5'-hydroxy-averantin
averufin
mutagenicity
