摘要
对牛密质Kiel骨的理化性能进行了研究,分析了Kiel骨的理化性能和其临床应用之间的关系,以便对Kiel骨的性能进行改进并寻找异种骨材料加工的新方法。采用牛腿骨骨干根据Salama报道的方法制备了Kiel骨,并对材料进行红外光谱(FTIR)、X射线衍射光谱(XRD)、差热扫描分析(DSC)、单轴拉伸力学性能测试。结果表明经过一系列处理后,Kiel骨的无机成分主要是与人骨天然成分相同的羟基磷灰石,同时这种加工方法对有机成分的脱除并不彻底,经H2O2处理后,胶原蛋白只发生变性而并没有被脱除,这种结构变化使Kiel骨的力学性能下降。Kiel骨由于脱除了主要的免疫原性物质,其它的弱抗原物质发生蛋白质变性,其免疫原性较弱,但由于蛋白质变性降低了它的力学强度,同时也影响了胶原蛋白的生理功能,使植入材料的成骨性能较差,影响了其在临床上的广泛应用,采用温和的脱蛋白剂替代强氧化剂能够改善Kiel骨的理化性能。
Xenograft bone such as Kiel bone has long been used as a grafting materials,but the results of Kiel bone application in clinic are still controversial due to it's some drawbacks. To investigate the novel procession method of xenograft bone, the physicochemical characterization of Kiel bone and it's relationship with the clinical application are studied. Kiel bone is prepared from bovine femoral cortical bone according to Salama's methods.The bone specimens are characterized with a number of physicochemical methods such as FTIR spectroscopy, X-ray diffraction spectroscopy , differential scanning calorimetry (DSC) and uniaxial tension mechanical tests. The result indicates that Kiel bone is composed of hydroxyapatite and organic component ,and the deproteination of Kiel bone is imcomplete, but processes with hydrogen peroxide, the collagen is denatured which reduce the mechanical properties of materials. The immunogenicity of Kiel bone is weakened since the main antigenic protein is removed and collagen is denatured, which not only weakens the strength of materials but influences on it's physiological property, all make the osteoinductive properties of materials unideal. To overcome these problems, it is necessary to develop the novel procession method of the xenogeneic bone.
出处
《重庆大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2004年第7期40-44,共5页
Journal of Chongqing University
基金
国家自然科学基金资助项目(30270395
19872082)
关键词
Kiel骨
制备
理化性能
Kiel bone
preparation
physicochemical characterization
