摘要
运用我所建立的双链DNA直接测序法测定了GMA诱导的质粒抗性基因片段(Tc^R)EcnRI/SaII片段(～0.3Kb)的序列,并与相应正常基因片段序列作一比较,所得结果具有一定意义。本实验表明:双链DNA直接测序法可应用于环境诱变剂研究。
The EcoRI/SalI fragment (~0.3Kb) from mutant ApRTcS of plasmid pBR322 induced by glycidyl methacrylate (GMA) has been sequenced after recom-binated with vector using directly sequencing for double stranded DNA. In most case the mutation occured in the form of deletion and insertion of cytidine and guanine to the sequenced fragment.The results showed that the method directly sequencing for double stranded DNA is useful to the study of environmental mutagens.
出处
《癌变·畸变·突变》
CAS
CSCD
1993年第1期26-28,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
双链DNA
直接测序
环境
诱变剂
GMA
Directly sequencing of double stranded DNA
Mutagenesis of environ-mental factor
GMA
