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多肽TAT与核定位信号介导的蛋白质入核递送 被引量：5

TAT Peptide and Nuclear Localization Signal Mediated Protein Delivery into Cell Nuclei
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摘要 增强型绿色荧光蛋白与蛋白质转导结构域TAT、SV40大T抗原的核定位信号以融合蛋白的形式在大肠杆菌中表达 ,纯化后转导A431细胞 ,大部分细胞核内都可以观察到绿色荧光 ,说明TAT NLS可以有效介导蛋白质的入核递送。这种蛋白质递送系统可望用于转录治疗等研究领域。 Enhanced green fluorescence protein was expressed in E.coli as a fusion protein with TAT peptide and the nuclear localization signal of SV40 large T antigen.When purified protein TAT-NLS-eGFP-His-6 was incubated with A431 cells,it was able to accumulate within most cells' nuclei.This type of protein delivery system may be of potential application as a tool for transcription therapy research.;
作者 吴国清 朱旭东 刘娟 党颖 李少林 黄培堂
机构地区 军事医学科学院生物工程研究所 重庆医科大学
出处 《中国生物工程杂志》 CAS CSCD 2004年第5期61-63,19,共4页 China Biotechnology
基金 国家"973"计划资助项目 ( 2 0 0 2CB5 13 2 0 4)
关键词 TAT 核定位信号 增强型绿色荧光蛋白 蛋白质转导结构域 细胞核 TAT NLS eGFP PTD
分类号 Q26 [生物学—细胞生物学]
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参考文献8

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二级参考文献14

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共引文献8

同被引文献52

  • 1李慧昕,王君伟,李宝臣,韩先杰,何海娟,李昭春.牛病毒性腹泻病毒P20和P14基因的克隆及序列分析[J].中国预防兽医学报,2005,27(2):124-129. 被引量：6
  • 2刘坤,姜颖,贺福初.脂筏在病毒感染中的作用[J].中国生物化学与分子生物学报,2006,22(10):767-771. 被引量：6
  • 3Wu SP,Fu AL,Wang YX,Yu LP,Jia PY,Li Q,Jin GZ,Sun MJ.A novel therapeutic approach to 6-OHDA-induced Parkinson＇s disease in rats via supplementation of PTD-conjugated tyrosine hydroxylase[J].中国生物学文摘,2006,20(10):33-33. 被引量：9
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引证文献5

二级引证文献1

中国生物工程杂志
2004年 第5期

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