摘要
目的寻求高效、快速体外培养人头皮毛乳头细胞的方法。方法将含有毛囊中下部的皮下脂肪剪碎,加入胶原酶Ⅰ进行消化,以吸管机械吹打帮助毛乳头游离后进行培养;对其贴壁率、细胞迁出率、工作强度、污染机会与显微解剖法、显微解剖加酶消化法进行比较。结果“一步酶消化法”能显著降低工作强度、减少污染机会,并保留了显微解剖加酶消化法促进毛乳头贴壁和细胞迁出的优点。结论“一步酶消化法”是一种高效、快速分离培养人头皮毛乳头细胞的方法。
Objective To seek an efficient and rapid method for isolating and culturing human scalp dermal papilla cells in vitro. Methods Subcutaneous fat with mid-to-lower portions of hair follicles were cut to pieces and digestived by collagenaseⅠ.The dermal papillae were released synchronously by blowing up the collagenase with pipette.Adhesion of dermal papillae, cell emigration, workload and frequency of contamination were compared with that of microdissection and microdissection with digestive treatment. Results This method reduced the workload and frequency of contamination greatly,and remained the advantages of microdissection with digestive treatment that improve adhesion of dermal papillae and cell emigration. Conclusion One step digestive treament is an efficient and rapid method to isolate and culture humane scalp dermal papilla cells.
出处
《中国美容医学》
CAS
2004年第3期264-266,共3页
Chinese Journal of Aesthetic Medicine
基金
国家自然科学基金资助项目(30170973)
广东省自然科学基金资助项目(010435)
