摘要
HPLC法测定了不同产地保济丸中厚朴酚浓度.考察了样品制备方法、流动相系统、检测波长等因素对分析结果的影响.色谱柱为HypersilODS(4 6mm×150mm)不锈钢柱;以甲醇-水(V∶V=30∶70)为流动相;流速1mL/min,检测波长为294nm;进样量10μL.研究结果表明厚朴酚和其它组分可达基线分离,厚朴酚质量浓度在8×10-2～8×10-1mg/mL内线性关系良好(r=0 9979).保济丸中厚朴酚的回收率为99 46%,RSD为1 78%.
A simple HPLC procedure was developed for the determination of magnolol in Po Chai Pills.The effects of sample preparation,the mobile phase and detection wavelength on the separation of the various components in samples were investigated:a 4.6mm*150mm Hypersil ODS column with a mobile phase consisting of methanol-H_2O (77:23).It was detected at 294nm. The results showed that Magnolol were separated from other components. The standard curve was linear over the concentration range of 8×10^(-2)～8×10^(-1) and the correlation coefficent was (0.997?9). The recoveries of magnolol were 99.46% for Po Chai Pills and RSD was 1.78%.
出处
《华南师范大学学报（自然科学版）》
CAS
2004年第2期90-94,共5页
Journal of South China Normal University(Natural Science Edition)
基金
广东省科技攻关资助项目(2002C30115)
