摘要
目的 了解易栓性疾病患者凝血因子Ⅴ基因三种多态性和狼疮抗凝物质(LA)、活化蛋白C抵抗(APC-R)的发生率。方法 测定237例易栓性疾病(包括90例脑梗塞、59例冠心病、57例高血压、31例深静脉血栓)患者和正常对照血浆中LA、APC-R,并用限制性内切酶片段多态性方法测定FV G1691→A、C1091→C、A1090→G三种基因多态性的发生情况。结果 脑梗塞组LA阳性者有41例(45.6％),APC-R阳性者3例(3.3％);冠心病组LA阳性者有24例(40.7％),APC-R未见阳性;高血压组LA阳性者有22例(38.6％),APC-R阳性者有4例(7.0％);深静脉血栓组LA阳性者有13例(41.9％),APC-R阳性者1例(3.2％);对照组未见阳性、三种基因多态性都未见阳性。结论 易栓性疾病患者LA阳性率明显高于正常对照,LA可能是引起易栓性疾病患者血栓形成的重要原因之一,易栓性疾病患者存在APC—R,但可能和凝血因子Ⅴ基因三种多态性无关。
Objective To evaluate the incidence of gene polymorphism of coagulation factor V and activated
protein C-resistance(APC-R)and lupus anticoagulant (LA) in patients with thrombophilia. Methods APC-R, LA
and FV G1691→G, A1090→G gene polymorphism were assasyed in 237 patients with thrombophilia and 60 nor-
mal subjects. Results There were 41 positive for LA (45.6%), 3 positive for APC-R(3.3%)in patients with Cere-
bral infarct; 24 positive for LA(40.7%), 0 positive for APC-R in patients with Coronary heart disease; 22 posi-
tive for LA (38.6%), 4 positive for APC-R(7.0%) in patients with high blood pressure; 13 positive for LA
(41.9%), 1 positive for APC-R (3.2%), in patients with deep vein thrombosis. There were no positive for LA
and APC-R in the control subjects. There were no FV G1691→A, G1091→C, A1090→G gene mutation in pa-
tients with thrombophilia. Conclusion There were especially high positive LA rate and perhaps LA is one of
the important causes of thrombosis in patients with thrombophilia. There were APC-R phenomenon in patients
with thrombophilia, perhaps having no relationship with factor V gene mutation.
出处
《海南医学》
CAS
2004年第6期35-36,24,共3页
Hainan Medical Journal
关键词
易栓性疾病
血浆
凝血因子V
基因多态性
APC-R
LA
狼疮抗凝物质
活化蛋白C抵抗
Cerebral infact
Coronary heart disease
High blood pressure
Deep vein thrombosis
Coagulation factor V Lupus anticoasgulant
Gene polymorphism
Activated protein C-resistance
