摘要
目的 探讨肝、脾棘球蚴囊周围纤维性囊壁的不同形成机制。 方法 苏木素 伊红染色 ,观察 40例肝棘球蚴囊、15例脾棘球蚴囊周围纤维囊壁及其邻近肝、脾实质病理组织学改变。免疫组织化学方法检测Ⅳ型胶原、纤维连接蛋白 (FN)、层粘连蛋白 (LN) ;原位杂交方法检测转化生长因子 β1(TGF β1)及肿瘤坏死因子 α (TNF α)的mRNA在肝、脾棘球蚴囊周围纤维囊壁及其邻近肝、脾实质中的表达。 结果 肝棘球蚴囊周围纤维囊壁分两层 ,虫体侧纤维囊壁为肉芽肿样组织 ,肝实质侧纤维囊壁内可见大量受挤压的门静脉、肝动脉和肝管系统 (Glisson)和肝静脉系统 ,并与Glisson鞘相延续。Ⅳ型胶原、FN、LN、TGF β1及TNF α在两层中的表达差异均有显著性意义 (P均 <0 0 1)。脾棘球蚴囊周围纤维囊壁不分层 ,为肉芽肿样组织 ,Ⅳ型胶原、FN、LN、TGF β1及TNF α无特异分层表达。 结论 肝、脾棘球蚴囊周围纤维囊壁的形成机制不同。肝棘球蚴囊周围纤维性囊壁是人体形成肉芽肿样病理改变后被周围受挤压的Glisson系统和肝静脉系统包裹 ,过度肝纤维化所致 ,肉芽肿样组织与周围纤维化的Glisson系统和肝静脉系统间有可分离间隙。脾棘球蚴囊周围纤维性囊壁是肉芽肿样组织包裹虫体形成 。
Objective To investigate the formative mechanisms of the fibrous capsule around hepatic and splenic hydatid cyst. MethodsHE stain was used to observe the histopathologic changes of the fibrous capsules around hepatic and splenic hydatid cysts and the adjacent parenchyma. The expression of collagen Ⅳ, fibronectin(FN), laminin(LN), transforming growth factor_β1 (TGF_β1) and tumor necrosis factor_α(TNF_α) were detected by using immunohistochemistry or in situ hybridization in fibrous capsules around 40 hepatic hydatid cysts and 15 splenic hydatid cysts including adjacent parenchyma. Results A special delaminated phenomenon was observed in the fibrous capsules around hepatic hydatid cysts. Granuloma_like pathologic changes were found in the fibrous capsules near worm and there were many depressed Glisson capsules and hepatic vein system in the fibrous capsules near hepatic parenchyma while these phenomena could not be observed in splenic hydatid cysts. All parameters were highly expressed in the fibrous capsules near hepatic parenchyma and the difference between the two fibrous capsules was significant(P<0^01). Conclusion The mechanisms of forming fibrous capsule around hepatic and splenic hydatid cysts are different. The fibrous capsule around hepatic hydatid cyst is a granuloma_like structure, covered by the pressed Glisson system and hepatic vein system with a small gap between them; while the fibrous capsule around splenic hydatid cyst is formed by granuloma_like tissue covering the worm and there is no gap between the capsule and the splenic parenchyma.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2004年第1期1-4,i005,共5页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家自然科学基金 (No 30 2 60 1 0 8)~~
