摘要
目的 :探讨小柴胡汤、丹那唑及二者联合用药对子宫内膜异位症 (EM)模型大鼠血管生成因子和异位内膜微血管密度的影响。方法 :EM模型大鼠随机分为不用药组 (U)、小柴胡汤 (XCH)、丹那唑组 (D)和联合用药组(S) ,设假手术组 (C)为对照。四周后观察各组异位子宫内膜体积和形态学的变化 ,计数腹腔液巨噬细胞 ,放射免疫法测定各组大鼠外周血、腹腔液及巨噬细胞培养液白细胞介素 - 8(IL - 8)、肿瘤坏死因子 (TNF -α)的含量 ,免疫组化法测定血管内皮生长因子 (VEGF)在异位内膜中的表达 ,并通过CD34标记异位子宫内膜血管 ,测定其微血管密度(MVD)。结果 :XCH组、D组和S组异位内膜呈现不同程度萎缩 ,腺体明显减少 ,腹腔液巨噬细胞计数减少 ,XCH组、D组及S组大鼠外周血、腹腔液及巨噬细胞培养液IL - 8、TNF -α含量降低 ,异位内膜VEGF表达减弱 ,MVD明显减少 ,其中以S组最为明显。结论 :小柴胡汤和丹那唑可以抑制EM模型大鼠异位内膜的血管生成 ,使异位内膜萎缩 ,当二者联合用药时 ,作用更强。
AIM: To investigate the effects of Xiaochaihutang (XCH), a Chinese medicine, and danazol on angiogenesis factor and microvessel density (MVD) of endometriosis (EM). METHODS: EM rats were treated with XCH, Danazol (D), and XCH plus D (group S) for 4 weeks, respectively. The histomorphology and volumes of ectopic endometrium were observed, the amount of macrophages in peritoneal fluid of EM model rats was counted, the concentration of interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) of EM model rats in serum, peritoneal fluid, and supernate of cultured macrophages were measured. In addition, vascular endothelial growth factor (VEGF) was detected in ectopic endometrium by immunohistochemical SABC technique, MVD was determined by immunostaining for CD34. Similar studies were performed in rats without treatment (U group) and another group with sham operation (C). RESULTS: Compared with U group, XCH group, D group, and S group displayed a significant atrophy of ectopic endometrium, reduced number of endometrial glands, decreased macrophage amounts and low concentrations of IL-8 and TNF-α in blood, peritoneal fluid, and supernate of cultured macrophages . The expression of VEGF and MVD of ectopic endometrium in U group were significantly higher than that of C group. After treatment, they all decreased significantly, especially in S group. CONCLUSIONS: Both XCH and danazol can affect angiogenesis of EM model rats, cause an obvious atrophy in ectopic endometrium. XCH in combination with danazol can enhance the inhibitory effect on angiogenesis of EM model rats.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第5期862-865,共4页
Chinese Journal of Pathophysiology
