摘要
〔目的〕为早期发现健康人群丙型肝炎感染状况 ,对漯河市无偿献血人员进行丙型肝炎病毒抗体 (抗 -HCV)检测 ,为安全输血制订丙型肝炎预防措施提供参考依据。〔方法〕采用酶联免疫法 (ELISA)与胶体金法两种方法进行比较。〔结果〕2年 ( 2 0 0 1-2 0 0 2年 )检测献血人员 1682 8人 ,ELISA法阳性 13 3份 ,阳性率 0 .79% ;胶体金法阳性 13 0份 ,阳性率 0 .77%。符合率 97.74% ,二者阳性结果无显著性差异 ( χ2 =0 .0 14 ,P >0 .0 5 )两种方法阴性对照结果一致 ,符合率10 0 %。〔结论〕胶体金法检测抗 -HCV ,具有操作简便 ,观察直观、快速、省时。其特异性、敏感性均达到ELISA水平。可作为筛查无偿献血人员丙型肝炎病毒抗体的重要手段。对丙型肝炎的早期发现 ,预防及控制有重要价值和意义。
Objective To find early the HCV infection in healthy individuals to provide basis for formulation of safe measures for blood donation.〔Methods〕The anti-HCV antibody in healthy individuals in Luohe City were detected with ELISA and colloidal gold method,respectively.〔Results〕 In the 2 years (2001-2002),the blood samples from 16828 healthy blood donors were tested with the 2 methods.The samples from 133 donors were positive for the detection with ELISA (positive rate=0.79%) and those from 130 donors were positive for the detection with colloidal gold method (positive rate=0.77%).There was no significant difference in the positive rate between the 2 methods (P>0.05).The conformity rate was 100%.〔Conclusions〕Compared with ELISA,the colloidal gold method is simpler,more direct,faster and more time-saving.Therefore,it can be used to detect HCV infection in blood donors.
出处
《中国卫生检验杂志》
CAS
2004年第1期21-21,共1页
Chinese Journal of Health Laboratory Technology
