摘要
类黄酮-3’,5’-羟基化酶(F3'5'H)是合成蓝色花色素的关键酶,近十多年一直都是蓝色花分子育种领域的研究热点之一。目前已从13种植物中分离到了F3'5'H基因。本文通过对F3'5'H基因已知氨基酸序列进行分析,列出了F3'5'H的功能基序;构建的系统进化树系图体现了各科属间的亲缘关系与进化差异;研究结果表明:不同物种F3'5'H基因的时空表达特性不同,与表达部位的发育进程和着色模式有关;作者还从底物特异性和遗传转化两个方面分析了该基因功能研究的进展。
Flaconoid-3',5'-hydroxylase (F3'5'H) was the key enzyme in the synthesis pathway of 3',5'-hydroxylated an-thocyanin (blue anthocyanidin) , which are generally required for the expression of blue or purple flower color. Therefore, the expression and regulation characterization of genes encoding F3'5'H is of great interest for blue flower molecular breeding in the last decade. Researches on the isolation and subsequent expression, function studies of F3'5'Hgenes has been reviewed in this paper. F3'5'H genes have been isolated from 13 plants. Based on the analysis of the known sequence of amino acid, the motif and phylogenetic tree are listed. The tree is a well embodiment of interspecies phylogenetic relationship and evolvement order. Spatio-temporal expression characteristic among species is different. Studies on the function of F3'5'Hhomologous genes are also been discussed.
出处
《分子植物育种》
CAS
CSCD
2004年第3期413-420,共8页
Molecular Plant Breeding
