摘要
目的 探讨硫氧化还原蛋白 (Trx)对胃癌细胞化疗敏感性的调节作用。方法 通过RT PCR从人胃癌耐药细胞系SGC790 1/VCR细胞中克隆Trx全长cDNA ,构建Trx正反义真核表达载体 ,并借助脂质体将正义载体转入胃癌细胞SGC790 1、反义载体转入胃癌耐药细胞SGC790 1/VCR ,利用MTT试验在体外检测基因转染细胞及其对照细胞对化疗药物的敏感性。结果 转染Trx正义载体后 ,SGC790 1细胞对长春新碱、顺铂、阿霉素的敏感性显著降低 ;转染Trx反义载体后 ,SGC790 1/VCR细胞对长春新碱、顺铂、阿霉素的敏感性显著升高。结论 Trx可能参与了胃癌细胞多药耐药性的形成 。
Objective To explore the regulatory effects of thioredoxin on chemosensitivity of gastric cancer cells. Methods RT-PCR was employed to clone the full-length cDNA of thioredoxin (Trx) from human drug-resistant gastric cancer cell line SGC7901/VCR. The sense and antisense eukaryotic expression vectors corresponding Trx were constructed and transduced into gastric cancer cells SGC7901 and SGC7901/VCR by lipofectamine, respectively. MTT assay was used to evaluate the sensitivity of gene-transfected gastric cancer cells and control cells to anti-cancer drugs in vitro. Results The results indicated that SGC7901 cells transfected with Trx sense vector showed significantly decreased sensitivity to vincristine, cisplatin and adriamycin, while SGC7901/VCR transfected with Trx antisense vector showed significantly increased sensitivity to these drugs. Conclusion All these data suggested that Trx might participate in the formation of multidrug resistance of gastric cancer cells and alteration of Trx could regulate chemosensitivity of gastric cancer cells.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第5期424-426,共3页
Medical Journal of Chinese People's Liberation Army
