摘要
目的 观察反义c fos核酸对自体移植血管平滑肌细胞表型的影响 ,从基因治疗角度来探讨血管移植后再狭窄的防治疗法。方法 选择 2 0只Wistar大鼠 ,利用显微外科技术建立自体静脉腹主动脉移植模型 ,随机等分为实验组和对照组 ,实验组对移植静脉血管进行反义c fos核酸溶液浸泡 ,并在吻合口周围涂布含反义c fos核酸的凝胶 ,术后 2周取出移植血管 ,利用电子显微镜观察平滑肌细胞增殖情况 ,利用免疫组织化学方法对移植血管进行c fos、c myc、PCNA表达检测。结果 实验组肌内皮结构清晰 ,对照组模糊不清 ,实验组增殖型血管平滑肌细胞数和PCNA、c fos、c myc阳性表达状态低于对照组。结论 反义c fos核酸可有效抑制移植静脉血管平滑肌细胞的增殖 。
Objective To investigate the effect of c-fos antisense oligonucleotide on the phenotypes of vascular smooth muscle cells in venous autograftt. Methods The external jugular veins were grafted into abdominal aortic arteries in 20 Wistar rats which were divided into test group and control group randomly. The anastomosis and transplanted veins were coated with c-fos antisense oligonucleotide glue gel in the test group while the control group were merely coated with glue gel. The autografted veins were removed and measured by means of pathology and immunohistochemistry two weeks later. Meanwhile the conversion status of the vascular smooth muscle cells were observed with electron microscope. Results The myo-endothelial structure was observed clearly in test group while it was obscure in control group; the expression of c-fos、c-myc、PCNA in vascular smooth muscle cells was significantly decreased in test group. Conclusions The c-fos antisense oligonucleotide can influence the phenotypes of the vascular smooth muscle cells in autografted veins and inhibit the cells’ proliferation. All these indicate that it is a prospective genetic prophylactic therapy for the restenosis of autografted veins.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第5期403-405,共3页
Medical Journal of Chinese People's Liberation Army
基金
中国博士后科学基金资助课题 (编号中博基 [1 999] 1 7)
关键词
C-FOS
反义核酸
血管平滑肌细胞
再狭窄
c-fos
antisense oligonucleotide
vascular smooth muscle cell
restenosis
