摘要
目的 :对脱氮污水处理工艺的活性污泥的菌群组成进行分析 ,以期获得适合于脱氮基因工程改良的出发菌。方法 :首先采用平板稀释法对活性污泥进行菌落计数 ,并对分离到的菌落进行详细的生化鉴定 ,对其中的优势菌—假单胞菌进行脱氮能力测定 ,并分析其对常作为筛选标志的抗生素的药物敏感性。结果 :发现在采用该工艺的活性污泥中 ,优势菌为假单胞菌、肠杆菌、莫拉菌和不动杆菌 ,分别占总菌数的2 3%、16 %、16 %和 12 %。根据菌群分析的结果 ,从中选择了两株耐药性弱、脱氮能力强的菌作为基因改良的出发菌。结论 :本研究阐明了活性污泥的菌群构成 ,获得了两株适合基因工程改良的菌株 ,为日后脱氮基因工程菌的构建奠定基础。
Objective:To explicate the composition of microflora in active sludge of sewage treatment.To obtain proper strains fitting for genes manipulating to enhance the ability of denitrification.Methods:First of all,we counted the population of bacteria in the active sludge.Using their biochemical and growth properties identify those strains we had isolated.Results:We noticed that dominative bacteria in the active sludge of denitrifying reactor were Pseudomonas(23%),Acinetobacter(12%),Moraxella(16%) and Enterobacter (16%).Chosing two of them based on their features about denitration and resistance to antibiotics.Conclusion:Those two bacteria were identified as Pseudomonas stutzeri and Pseudomonas mendocina.
出处
《中国微生态学杂志》
CAS
CSCD
2004年第2期83-84,共2页
Chinese Journal of Microecology
