摘要
目的 探讨新疆维吾尔族、汉族人群中谷胱甘肽S 转移酶 (glutathioneS transferase,GST)M 1、T1纯合缺失基因型 (- / - )与子宫内膜异位症 (内异症 )发病的关系。方法 采用PCR技术 ,分别对维吾尔族 10 7例正常妇女 (维族对照组 )及 4 1例内异症患者 (维族内异症组 )、汉族 10 5例正常妇女 (汉族对照组 )及 80例内异症患者 (汉族内异症组 )基因组DNA进行GSTM1、GSTT1基因分型。结果 维族内异症组GSTM 1(- / - )、GSTT1(- / - )基因型及两种基因同时缺失的基因型频率分别为 5 1 2 %、36 6 %、2 4 4 % ,维族对照组为 5 3 2 %、2 9 9%、13 1% ,两组比较 ,差异无显著性(P >0 0 5 )。汉族内异症组与汉族对照组GSTM1(- / - )基因型频率分别为 5 6 8%、5 1 8% ,两组比较 ,差异也无显著性 (P >0 0 5 ) ;而GSTT1(- / - )基因型频率分别为 73 7%、4 4 3% ,两组比较 ,差异有显著性 (P <0 0 5 ) ;汉族内异症组、汉族对照组两种基因同时缺失的基因型频率为 4 2 5 %、2 2 8% ,两组比较 ,差异有显著性 (P <0 0 5 )。维族与汉族比较 ,对照组GSTM 1(- / - )、GSTT1(- /- )基因型及两种基因同时缺失的基因型比较 ,差异无显著性 (P >0 0 5 ) ;内异症组GSTM 1(- / - )、两种基因同时缺失的基因型比较 ,差异也无?
Objective To examine the association between glutathione S-transferase (GST)M1,T1 null genotypes and endometriosis of the Uygurs and Hans in Xinjiang. Methods The polymerase chain reaction method was used to detect the presence or absence of the GSTM1 and GSTT1 genes in genomic DNA from the Uygurs (107 controls and 41 cases) and the Hans (105 controls and 80 cases) in Xinjiang. Results The frequencies of the GSTM1-null genotype, GSTT1-null genotype, combined GSTM1-null and GSTT1-null genotype in endometriosis of the Uygurs (51.2%,36.6%,24.4%) were not significantly different from those in controls(53.2%,29.9%,13.1%). Similarly, no statistically significant difference was observed in the frequency of GSTM1-null genotype in cases of endometriosis of the Hans (56.8%) compared with the controls(51.8%),but the frequencies of GSTT1-null genotype, combined GSTM1-null and GSTT1-null genotype in endometriosis of the Hans(73.7%,42.5%) were significantly different from those in controls(44.3%,22.8%). When comparing the Uygurs with the Hans, we found no significant difference in the frequencies of GSTM1-null genotype,GSTT1-null genotype, combined GSTM1-null and GSTT1-null genotype between the two control populations, neither in the frequencies of the GSTM1-null genotype, combined GSTM1-null and GSTT1-null genotype between the two endometriosis populations. However, the frequency of GSTT1-null genotype in cases of the Hans(73.7%) was significantly higher than that in cases of the Uygurs(36.6%). Conclusions No evidence was found to suggest an association between GSTM1-null genotype and endometriosis in the Hans and Uygurs. An association was found between GSTT1-null genotype and endometriosis in the Hans, but not in the Uygurs. The two nationalities have different genetic predisposing factors to the development of endometriosis.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2004年第2期101-104,共4页
Chinese Journal of Obstetrics and Gynecology
