摘要
目的 研究转化生长因子 β1(transforminggrowthfactor β1,TGF β1)、即刻早期基因 (c fos)、周期素依赖性激酶抑制蛋白 p2 7在中耳胆脂瘤上皮中的表达及相互之间的关系 ,探讨其表达对胆脂瘤侵袭能力的影响。方法 应用免疫组化链霉菌抗生物素蛋白 过氧化酶染色法检测TGF β1、c fos和 p2 7在 31例胆脂瘤上皮、11例胆脂瘤患者外耳道上皮和 10例正常外耳道上皮中的表达 ,应用计算机图像分析系统对其阳性表达进行定量分析。结果 TGF β1和c fos在胆脂瘤上皮中表达阳性率分别为 87 1%和 83 9% ,与胆脂瘤患者外耳道上皮和正常外耳道上皮相比表达差异有显著性。而p2 7在胆脂瘤上皮和外耳道上皮组上皮中均未见阳性表达。胆脂瘤上皮中c fos与TGF β1表达无相关性 (r =0 339,P =0 331) ;胆脂瘤侵袭能力与c fos表达有高度显著相关关系 (r =- 0 96 5 ,P <0 0 1) ,与TGF β1表达也有高度相关关系 (r =- 0 4 0 6 ,P <0 0 1)。 结论 即刻早期基因c fos在胆脂瘤中表达显著增强 ,且与胆脂瘤的侵袭能力有高度显著相关性 ,提示高c fos表达是胆脂瘤高增殖特征的因素之一。TGF
Objectives To analyze the expression of transforming growth factor β1 (TGF β1)、cyclin dependent kinase inhibitor(p27)and c fos in human middle ear cholesteatomas and to investigate the correlation between their expression and the ability of erosion of cholesteatoma. Methods Immunohisto chemical staining (SP method) of 31 cholesteatomas and 11 external ear canal skin samples from patients and 10 external ear canal skin samples from healthful men which were taken intraoperatively, was performed for c fos, TGF β1 and p27 positivity. The signals representing the expression of c fos、TGF β1and p27 were observed under microscope and scanned into a computer by an image scanner. The gray scale of positive signals were quantitated by image processing computer. Results The percentage of positive expression of TGF β1 and c fos in cholesteatoma were 87 1% and 83 9%, respectively. Their expression tended to be increased greatly compared with which in the skins of the control groups. Positive p27 signals were not observed in cholesteatomas and external ear skin tissues. It showed statistically significant correlation between expression of c fos and the ability of erosion of cholesteatoma. There was correlation between the express ion of TGF β1 and the ability of erosion of cholesteatoma too. But there was no correlation between the expression of c fos and TGF β1. Conclusion The expression of c fos in cholesteatoma was significally higher compared with which in the skin of external auditory of cholesteatoma patients and healthful men, which indicate that c fos plays an important role in the hyperproliferative of cholesteatoma. The expression of TGF β1 was significant higher in cholesteatoma, which indicate that cytokines such as TGF β1 play a great role in the etiology of cholesteatoma.
出处
《中华耳鼻咽喉科杂志》
CSCD
北大核心
2004年第4期211-215,共5页
Chinese Journal of Otorhinolaryngology
基金
云南省卫生厅中西医结合科研基金资助 (2 0 0 2 0 2 )
