摘要
目的 :研究诱导型一氧化氮合酶(iNOS)选择性抑制剂氨基胍 (aminoguanidine ,AG)胃癌细胞增殖效果。方法 :采用MTT法观察AG在不同时间点对MFC细胞增殖的影响 ,Greiss反应法检测对细胞培养上清液中一氧化氮 (NO)含量的影响。应用HE和免疫组化 (SP法 )染色 ,计数肿瘤组织中微血管密度 (MVD)和iNOS阳性率 ,并分析其与AG抑瘤效应的关系。结果 :1)在 0 1～ 10 0mmol/L浓度下 ,AG对MFC细胞生长抑制率与 0 0 0 1mmol/LAG比较 ,P <0 0 1;AG对MFC细胞合成NO具有明显的抑制作用 (与 0mmol/LAG比较 ,P <0 0 1) ,但其作用并不呈时间依赖性。 2 )大、中、小剂量AG对小鼠胃癌移植瘤的抑瘤率分别为5 2 9%、47 1%和 3 5 3 %。MVD阳性率AG大 、AG中 组同对照组比较差异有统计学意义 ,P <0 0 1。iNOS阳性率AG大 、AG中 组同对照组比较差异有统计学意义 ,P <0 0 1。结论 :AG在体外通过阻断NO途径显著抑制MFC胃癌细胞的生长 ,在体内明显抑制小鼠胃癌移植瘤的生长和肿瘤组织中iNOS表达 ,并且能显著降低MVD。
OBJECTIVE:To investigate the inhibition effects of aminoguanidine (AG),a selective inhibitor of inducible nitric oxide synthase,on the proliferation of MFC stomach cancer cell line and therapeutic effects on the implanted stomach carcinoma. METHODS: MTT method was used to examine the effects of AG on the proliferation of MFC cell lines. Nitrite levels in MFC cells supernatants were tested by using the Greiss assay.Inhibition rates, intratumoral microvessel density (MVD), the positive rate of inducible nitric oxide synthase were evaluated respectively after the mice were sacrificed. RESULTS: 1)Over a concentration ranged from 0.1 to 100 mmol/L,AG had inhibited the growth of MFC cell significantly. Nitrite levels in MFC cells supernatants were evidently inhibited.2) The growth of implanted tumor of MFC stomach cancer was inhibited by 52.9%, 47.1% and 35.3% at groups treated with high, middle and low dosage of AG,respectively. The MVD was decreased significantly in treated group. The positive rate of iNOS in treated group was lower than that in control group. CONCLUSIONS: AG can significantly inhibit the proliferation of MFC in vitro and the growth of implanted stomach carcinoma mice in vivo,and also inhibit the expression of iNOS and decrease MVD in carcinoma biopsy.
出处
《肿瘤防治杂志》
CAS
2004年第3期252-255,共4页
China Journal of Cancer Prevention and Treatment
基金
吉林省自然科学发展基金资助项目 ( 2 0 0 10 5 3 6)
关键词
一氧化氮合酶/治疗应用
胃肿瘤/病理学
胃肿瘤/药物疗法
肿瘤
实验性
nitric oxide synthase/therapeutic use
stomach neoplasms /pathology
stomach neoplasms / drug therapy
neoplasms, experimental
