摘要
目的 :探讨射频消融治疗对肺癌患者免疫功能的影响 .方法 :采用流式细胞术对 89例肺癌患者射频消融术前、后免疫活性细胞进行监测 .结果 :射频治疗前T细胞CD3+ ,CD4 + ,CD8+ 及NK细胞CD1 6 + 处于一个较低水平 ,分别为[(6 3.32± 3.0 6 ) ,(36 .83± 1 .32 ) ,(2 3.7± 1 5 .0 1 ) ,(1 6 .80±1 0 .90 ) ],B细胞CD1 9+ 亦处在较低水平 [(1 6 .30± 9.81 ) ];射频治疗后T细胞CD3+ ,CD4 + ,CD8+ 显著增高分别为 ,[(71 .33± 1 9.77) ,(4 6 .2 0± 1 1 .30 ) ,(30 .6 0± 1 0 .98) ]与正常组比较无统计学意义 ,NK细胞和B细胞明显增高 [(2 0 .30± 8.1 2 ) ,(1 9.90± 1 1 .4 0 ) ].T细胞CD4 + /CD8+ 比值治疗前与正常对照组对比无统计学差异 ,治疗后比值与正常组对比显著增高 [(1 .6 3± 0 .5 3)vs(4 .35± 1 .89)P <0 .0 5 ]并超出正常范围 .结论 :肺癌患者特别是晚期肺癌患者细胞免疫功能处于抑制状态 ,体液免疫功能亢进 ;射频消融治疗后激发了机体细胞免疫功能 .
AIM: To explore the effects of radiofrequency hyperthermia on the immune function of patients with lung cancer. METHODS: Immune function of 89 cases of patients with lung cancer was assessed by means of flow cytometry before and after radiofrequency hyperthermia. RESULTS: The numbers of CD3 +,CD4 +,CD8 + and natural killer cells (NK) CD16 + decreased obviously and the number of B cells CD19 + increased significantly before radiofrequency hyperthermia [(63.32±3.06), (36.83±1.32), (23.7±15.01), (16.80±10.90), (16.30±9.81)]. After radiofrequency, the number of CD3 + (71.33±19.77),CD4 + (46.20±11.30),CD8 + (30.60±10.98) and T cells increased distinctly, close to the normal level. NK cells (20.30±8.12) and B cells (19.90±11.40), increased obviously. The ratio of CD4 +/CD8 + T cells had no difference from that of normal control before treatment, but increased sharply after treatment and [(1.63±0.53) vs (4.35± 1.89) P <0.05] and exceeded the normal range. CONCLUSION: The cellular immune function in patients with lung cancer is inhibited, especially in advanced stage, but the humoral immunity is at the hyperfunctional state. Radiofrequency hyperthermia accounts for the activation of the immune function in patients with lung cancer.
出处
《第四军医大学学报》
北大核心
2004年第7期671-672,共2页
Journal of the Fourth Military Medical University
关键词
射频消融
肺肿瘤
T淋巴细胞群
B淋巴细胞
自然杀伤细胞
流式细胞术
radioferquency ablation
lung neoplasms
T lymphocyte subsets
natural killer cell
B lymphocyte
flow cytometry
