摘要
目的 :观察尾加压素Ⅱ (UⅡ )对心肌细胞 (MC)肥大的影响及其与钙离子的关系 ,为探讨高血压左室肥厚发生机制提供理论依据 .方法 :以培养的SD乳鼠心肌细胞为实验模型 ,通过测定MC表面积、蛋白含量和蛋白合成速率 ,以及免疫荧光观察MC内肌原纤维的变化趋势来探讨UⅡ对MC肥大的影响 .结果 :①随着UⅡ浓度的增加 ,MC表面积明显增加 ,呈剂量依赖性 ,其中 1 0 -8和 1 0 -7mol/LUⅡ组MC表面积分别为 (2 0 4 6 .3± 2 6 8.4 )和 (36 6 2 .2± 2 5 9.2 ) μm2 ,均明显高于空白对照组的 (936 .2± 99.8) μm2 ,差异有非常显著意义 (P <0 .0 1 ) .②随着UⅡ浓度的增加 ,MC的蛋白含量和蛋白合成速率呈递增趋势 ,其中 1 0 -8和 1 0 -7mol/LUⅡ组MC蛋白含量分别为 (1 .6 0± 0 .37)和 (1 .89± 0 .2 5 )ng/cell,明显高于对照组(0 .83± 0 .1 6 )ng/cell,差异有非常显著性意义 (P <0 .0 1 ) .蛋白合成速率分别为 (2 5 0 8.33± 2 99.81 )和 (2 898.83± 6 2 5 .4 1 )cpm/well,与对照组 (1 0 2 6 .83± 91 .6 7)cpm/well比较 ,差异非常显著 (P <0 .0 1 ) .③ 1 0 -7mol/LUⅡ作用心肌细胞 2 4h后 ,荧光显微镜下观察到细胞边界增大 ,肌原纤维发生增粗与重排 .结论 :UⅡ能够诱导心肌细胞的肥大 ,为研究高血压及其他心血管?
AIM: To observe the hypertrophy effects induced by urotensinⅡ (UⅡ) on the cultured cardiomyocytes and its relation with calcium. METHODS: The effects of UⅡon cardiac myocyte (MC) hypertrophy was assessed by the measurement of cell surface and 3H leucine incorporation and FITC conjugated phalloidin immunocytochemistry. RESULTS: ① UⅡ increased MC surface area in a dose dependent manner. In the groups with 10 -8 and 10 -7 mol/L UⅡ,the cell surface of MC was (2046.3±268.4) and (3662.2±259.2) μm 2, respectively, both of which were significantly higher than that of control( P <0.01).②UⅡ also increased the 3H leucine incorporation in a dose dependent manner, the protein content of 10 -8 and 10 -7 mol/L UⅡ groups being (1.60±0.37) and (1.89±0.25) ng/cell respectively and the 3H leucine incorporation was (2508.33±299.81) and (2898.83±625.41) cpm/ well, respectively. They were all significantly higher than those of control( P <0.01).③Compared with the control, the enhanced sarcomere organization was seen in MC stimulated by 10 -7 mol/L UⅡ. CONCLUSION: UⅡcan induce MC hypertrophy, which can be useful for the investigation of left ventricular hypertrophy induced by hypertension and other cardiac diseases.
出处
《第四军医大学学报》
北大核心
2004年第7期641-644,共4页
Journal of the Fourth Military Medical University
关键词
尾加压素Ⅱ
心肌细胞
肥大
urotensinⅡ
cardiac myocyte
hypertrophy
