摘要
本研究对6例Reiter综合征(Reiter’s syndrome,RS)患者进行大便培养,从1例RS患者中分离得到福氏志贺氏菌(Shigella Flexneri,SF,代号SF22682)。研究发现,SF22682所含的3kb质粒DNA可与代表HLA-B27分子片段的15mer DNA探针出现阳性杂交反应,而其它非来源于RS患者的多种SF,对照痢疾杆菌及大肠杆菌质粒DNA则为阴性,提示SF22682的3 kb质粒DNA编码的多肽可能通过与B27分子在分子水平的模拟而在诱发B27阳性个体发生RS上起重要作用。本文还简要讨论了SF的3kb质粒DNA在RS发病机制中的可能作用。
Reiter's Syndrome (RS) is well-known as a form of arthritis that develops notoriously in HLA-B27 positive subjects and Shigella Flexneri (SF) has long been proposed as the responsible agent of postdysenteric RS. In the present study, a strain of SF (designated as SF22682) was identified from one of the six RS patients on two occasions. Southern and dot hybridization was carried out between plasmid DNA extracted from SF22682 along with several other strains of SF and a synthesized 15 mer DNA probe. It was found that the probe hybridized only to a 3Kb pasmid DNA of SF22682. Since there is sequence homology between 15 mer DNA probe inferred pentapeptide and HLA-B27 residues, our results suggested that the 3Kb plasmid DNA might encode protein products showing sequence homology with HLA-B27 molecule and thus implicated in the pathogenesis of RS.
出处
《军医进修学院学报》
CAS
1992年第4期290-293,共4页
Academic Journal of Pla Postgraduate Medical School
