摘要
目的 :观察 8Hz,130dB次声暴露不同时间对大鼠脾、肝脏某些酶活性的影响。方法 :35只SD大鼠随机分为5组 ,即对照组 ,1周 ,2周 ,3周 ,4周组。每天次声暴露 1次 ,每次 2h。实验后 ,观察大鼠脾、肝脏组织中MAO ,GSH - px ,SOD活性和MDA含量的变化。结果 :大鼠脾脏MAO活性 1周 ,2周时显著增高 (P <0 .0 1) ,3周下降 ,4周时又显著增加 (P <0 .0 5 )。肝脏组织MAO活性变化不明显 (P >0 .0 5 )。脾脏组织中GSH px活性在 4周时明显增高 (P <0 .0 5 ) ,肝脏组织中GSH px活性在 1周时就有显著性增高 (P <0 .0 5 )。脾脏SOD活性在 1周至 4周均有显著性增高 (P <0 .0 5 )。肝脏组织在实验期变化不明显 (P >0 .0 5 )。脾脏组织中MDA含量在 3周至 4周时有显著性增高 (P <0 .0 5 )。肝脏组织在 1至 2周时有非常显著的增高 (P <0 .0 1) ,在 3周时下降 ,到 4周时又显著高于对照组 (P <0 .0 5 )。结论 :8Hz ,130dB次声暴露 ,大鼠脾、肝脏组织活性氧自由基、脂质过氧化物增高 ,抗氧化能力降低 ,造成对组织的损伤。
Aim: To investigate the changes of several enzymes activities in the spleen and liver of rats after exposure to 8 Hz 130 dB infrasound for different time. Methods: Thirty-five male SD rats were randomly divided into five groups. Rats of group 1 served as control, rats from group 2 to 5 were exposed to 8 Hz 130 dB infrasound, 2 hours per day, for 1 wk, 2 wk, 3 wk, and 4 wk, respectively. The changes of enzymes activities in spleen and liver of rats were observed. Results: Monoamine oxidase activities in spleen were significantly increased at 1 wk and 2 wk, it was decreased at 3 wk, and increased again at 4 wk (P<0.05). There were no changes in the liver compared with the control group. Glutathione peroxides activities in spleen were significantly increased at 4 wk(P<0.05) and it also increased in liver at 1 wk (P<0.05). Superoxide dismutase activities in spleen were increased significantly from 1 wk to 4 wk, but there were no markedly changes in liver. The level of malondialdehyde in spleen were increased at 3 wk and 4 wk. In the liver, it were increased at 1 wk an 2 wk, and decreased at 3 wk, but it increased again at 4 wk (P<0.05). Conclusion: The results indicated that lipid peroxidation and oxygen free radicals in spleen and liver were increased after infrasound exposure and it might induce the damage in tissue or cells.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2004年第2期176-179,共4页
Chinese Journal of Applied Physiology
基金
全军医学科学技术研究"十五"计划指令性课题( 0 1L0 71)
关键词
次声
脾脏
肝脏
酶活性
大鼠
应激
infrasound
spleen
liver
enzymes activity
rats
