摘要
目的 观察1 37Csγ射线照射对体外培养成骨细胞的形态、核浆比与几种细胞因子基因表达的影响。方法 取第 1代新生SD大鼠头盖骨成骨细胞传代后 2 4h一次接受1 37Csγ射线照射 ,吸收剂量分别为 10 ,2 5 ,5 0cGy和 1,3,6 ,9,12Gy。观察各组成骨细胞形态与超微结构改变 ;Giemsa染色后进行显微形态计量 ,计算核浆比 ;细胞培养至 14d抽提细胞内总RNA ,RT PCR方法半定量检测各组细胞IGF 1,OPG ,ODF基因mRNA表达量。结果 3Gy以上γ射线照射的细胞 ,胞体变大、变薄 ,细胞数减少 ,细胞内细胞器减少 ,溶酶体增多 ,核浆比降低 ,其改变随吸收剂量增加而突出。基因IGF 1、OPG、ODF与OPG ODFmRNA表达量在 1Gy以上组随吸收剂量增加逐渐降低 ,与吸收剂量呈明显相关 (P <0 0 5或 0 0 1)。结论 1 37Csγ射线照射引起成骨细胞形态明显改变 ,增殖能力降低 ,核浆比降低 ,基因IGF 1、OPG、ODF与OPG ODFmRNA表达量降低。
Objective To observe the influence of 137 Cs γ-irradiation on morphology and gene expression of osteoblasts cutured in vitro . Methods Osteoblasts were isolated from new-born SD rat crania and cultured in EMEM containing 10% NCS.The osteoblasts of the first passage were irradiated once with 137 Cs γ-rays after cultured for 24 hours.The irradiation doses were 10,25,50 cGy and 1,3,6,9,12 Gy,respectively.Their morphology and ultrastructure were observed under phase-contrast microscope and electronmicroscope.Karyoplasm ratio was determined after Giemsa staining.Total RNA was extracted with TRIzol reagent when the osteoblasts of the first passage were cultured for 14 days.mRNA expressions of insulin-like growth factor-1(IGF-1),osteoprotegerin(OPG) and osteoclast differentiation factor(ODF) were determined with RT-PCR semi-quantitatively and compared among different groups. Results The osteoblasts irradiated with 137 Cs γ-rays over 3 Gy were larger,thinner,containing more nuclei,proliferating slowly, having fewer cell organelles and more lysosomes in the plasma,with decreased ratio of nucleus to plasma.All of the above changes were more obvious with increasing dosage of irradiation.Quantity of IGF-1,OPG,ODF and OPG/ODF mRNA expressions were decreased with increasing dosage of irradiation above 1 Gy.It was obviously correlated with the quantity of irradiation ( P <0.05 or 0.01,respectively). Conclusion By 137 Cs γ-irradiation osteoblasts can be induced to have morphological changes,decreased ability of proliferation,decreased karyoplasms ratio and decreased quantity of IGF-1,OPG,ODF and OPG/ODF mRNA expressions. ;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2004年第1期26-29,共4页
Chinese Journal of Radiological Medicine and Protection
基金
国家教委博士点科研基金资助项目 (993 6)
