摘要
目的:对川白芷生长过程中不同形态的4个类群及杭白芷进行基因组DNA多态性分析,试图从分子水平为其栽培及种质鉴定提供依据。方法:以40个10碱基随机引物进行RAPD分析。结果:共扩增出314个条带,其中多态性条带96条,占31%。结论:川白芷叶柄颜色的不同与是否抽薹无明显的相关性,而产地的差异对遗传变异的影响大于因其叶柄颜色不同所造成的影响。
Objective: To analyze DNA polymorphism of the genomes of four populations with different forms of Radix Angelicae dahuricae in growth, trying to provide a basis for their cultivation and germenplasm identification at the molecular level. Method: To analyze RAPD by 40 random primers of 10 basic groups. Result: 314 bands have emerged, of which 96 are of polymorphism, accounting for 31%. Conclusion: There is no marked relativity between the difference of colours of the leaf stalks of Radix Angelicae dahuricae and its bolting. The influence of regions in which it grows on its hereditary variation is, however, greater than the influence exerted by the difference of colours of its leaf stalks.
出处
《世界科学技术-中医药现代化》
2004年第2期76-79,88,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
四川省科技厅资助项目(川科农犤2001犦22号):川白芷的规范化种植研究及基地建设
