摘要
观察同型半胱氨酸对血管平滑肌细胞基质金属蛋白酶 1活性的影响 ,并探讨其作用机制。采用体外培养的大鼠血管平滑肌细胞 ,加入不同浓度的同型半胱氨酸作用 4 8h ,通过酶谱分析和半定量逆转录聚合酶链反应的方法观察同型半胱氨酸对基质金属蛋白酶 1活性及其mRNA表达的影响。结果发现 ,同型半胱氨酸抑制基质金属蛋白酶 1活性 ,下调其mRNA表达 ,并有剂量依赖效应。提示同型半胱氨酸通过减少基质金属蛋白酶 1的合成 ,抑制其活性 ,从而减少胶原降解而使胶原蓄积。表明同型半胱氨酸减少胶原降解可能是致动脉粥样硬化的发病机制之一。
Aim To explore the effects of homocysteine (Hcy) on the activity of matrix metalloproteinase-1 (MMP-1) and it's regulatory mechanism in cultured vascular smooth muscle cells. Methods Cultured rat vascular smooth muscle cells were treated with different concentration of Hcy in vitro for 48 h. The activity of MMP-1 was determined using the method of zymography. The expression of MMP-1 mRNA in vascular smooth muscle cells was observed by semi-quantitative reverse transcription polymerase chain reaction. Results Hcy significantly inhibited the activity of MMP-1 and down-regulated the MMP-1 mRNA expression in vascular smooth muscle cells in a dose-dependent manner. Conclusion These data suggested that Hcy can induce collagen accumulation by decreasing the secretion of MMP-1 and inhibiting the activity of MMP-1. It may be the key factor in the pathogenesis of atherosclerosis induced by Hcy.
出处
《中国动脉硬化杂志》
CAS
CSCD
2004年第1期15-18,共4页
Chinese Journal of Arteriosclerosis
基金
教育部留学归国人员启动资金 ( 1999年 3 63号 )资助
