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热应激肉仔鸡胸腺、法氏囊超微组织学观察和细胞凋亡检测 被引量:19

Observations on Ultra Structure and Assaying Apoptotic Cells of Thymus and Bursa in the Experimental Heat-Stressed Broilers
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摘要 对试验性热应激肉仔鸡的胸腺、法氏囊的超微组织学结构进行了观察,并进行了细胞凋亡的检测。结果表明,通过电子显微镜观察,热应激前3d胸腺和法氏囊部分淋巴细胞凋亡,热应激5d后胸腺和法氏囊的淋巴细胞以坏死性变化为主。通过Tunel法检测,发现在热应激前3d,胸腺和法氏囊的细胞凋亡呈现逐渐下降的趋势。 The ultra-structure of thymus and bursa in the experimental heat-stressed broiders were observed by transmission electron microscope and the apoptotic cells were assaied by TUNEL with Deadend^(TM) colorimetric apoptosis detection system.The results showed that in the forepart time in the experimental heat stress lymphocytes of thymus and bursa showed obvious apoptotic character,then in the latter part time the cells mostly showed necrosis.The apoptotic lymphocyte in thymus and bursa only appeared 3 days before,there were no apoptotic after 5 days.The apoptotic indexes were gradually descended.
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2004年第3期310-313,共4页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 山东省教育委员会资助项目(J99H06)
关键词 热应激 肉仔鸡 胸腺 法氏囊 超微组织学 细胞凋亡 免疫功能抑制 Broiler Heat stress Thymus Bursa Ultra Structure Apoptosis
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参考文献11

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