摘要
经丙酮沉淀、UltrogelAcA5 4凝胶过滤、Q Sepharose离子交换柱层析和聚丙烯酰胺凝胶制备电泳 ,发现桑粒肩天牛幼虫肠液中具有所有水解纤维素成分的三种消化酶 ,并且每种酶都有不同数目的同工酶。纯化出一种内切 - β- 1 ,4 -葡聚糖酶 (EG - 1 ) ,其分子量为 2 6ku ,等电点约为pI4 0。酶的最适反应温度为 4 5℃ ,最适反应pH为5 2 ;不耐热 ,5 0℃处理 30min ,酶活大大降低 ,70℃处理 2h ,几乎丧失全部活性 ,显示出该酶适合动物肠道反应环境 ,是内源性酶。
One endo-β-1,4-glucanase (EG-1) was purified to electrophoretical homogeneity from the gut extract of Apriona germari larvae by acetone precipitating, gel filtrating, ion exchange chromatography and preparative PAGE, and its enzymatic properties were investigated. The enzyme component showed high activity on sodium carboxymethylcellulose (CMC). The molecular weight of EG-1 was 26?ku and the isoelectric point (pI) was about 4 0 It had an optimal temperature of 45℃ and optimal pH of 5 2 The enzyme activity was stable at 37℃ for at least 2?h, but decreased rapidly when kept at 50℃ and almost no activity was left after incubated at 70℃ for 2?h. All these showed that EG-1component was suit for the insect intestinal environment, and should be a endogenous enzyme.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2004年第2期103-106,共4页
Scientia Silvae Sinicae
基金
国家自然科学基金资助 ( 3 9770 62 1)
