摘要
利用中国春ph1b、ph2a、ph2b基因系及对照中国春分别与甘肃黑麦杂交,结实率分别为94.0％、87.9％、93.8％和90.8％,其F_1减数分裂中期Ⅰ染色体配对交叉数分别为9.748、2.968、5.000和1.376,ph1b、ph2a、ph2b基因诱导小麦与黑麦F_1部分同源染色体配对顺序是ph1b>ph2b>ph2a。用中国春回交F_1取得了成功,回交结实率分别为1.06％、0.73％、2.52％和11.40％。利用ph1b、ph2b基因可以将黑麦中有益基因直接遗传转移给小麦,ph2a在导入黑麦有益基因方面不宜利用,或其效果不及ph1b、ph2b,回交结实率与染色体配对有关。
‘Gan Su’rye was crossed with ‘Chinese Spring’mutants ph1b、ph2a、ph2b andwith ‘Chinese Spring’ as the control. The seed sets were 94.0%、87.9%、93.8% and90.8%, respectively, indicating no significant difference among them. Chiasma fre-quencies of the F_1 hybrids at meiotic metaphase I were 9.784、2.968、5.000 and1.376, respectively. The order of the ability of inducing homoeologous pairing by theph genes in wheat x rye F_1 hybrids at meiotic metaphase I is ph1b>ph2b>ph2a.Backcrosses of F_1 hybrids to Chinese Spring were successful and the backcrossesseed sets were 1.06%、0.73%、2.52% and 11.40%, respectively, indicating therewas significant difference between the ph derivatives and the control. So, directgenetic transfer of desirable genes from rye to wheat by ph1b、ph2b genes isavailable, but it is impossible by ph2a gene. Seed sets of backcrossing to the F_1hybrids are related to the homoeologous pairing at meiotic metaphase Ⅰ.
出处
《作物学报》
CAS
CSCD
北大核心
1992年第6期447-452,共6页
Acta Agronomica Sinica
关键词
ph基因系
黑麦
染色体交叉数
回交
ph lines
S. cereale
Homoeologous pairing
Chiasma frequency
Backcross
