摘要
微管蛋白 (tubulin)是一蛋白质超家族 ,其中α ,β 微管蛋白是主要的微管蛋白 ,而γ 微管蛋白主要在微管组装中起作用 .我们利用蛋白质印迹和激光共聚焦技术研究了γ 微管蛋白在猪卵母细胞成熟、受精和活化中的分布 .γ 微管蛋白存在于猪卵母细胞中 ,并且在减数分裂成熟各个时期的量保持不变 .它聚集在微管上 ,特别是中期纺锤体的两极和后末期的中板 .体外受精和孤雌活化后 ,γ 微管蛋白聚集在雌雄原核的周围 .另外它也存在于精子的顶体帽和颈部 .在早期卵裂中 ,γ 微管蛋白聚集在胚胎的细胞核周围 .实验结果表明 ,γ 微管蛋白在猪卵母细胞、精子和胚胎的微管组装中起重要的调节作用 ,在猪受精过程中 ,精子和卵子都向受精卵贡献中心体物质 .
Tubulin is one superfamily of proteins. α- and β-tubulins are the major proteins of microtubules, while γ-tubulin plays a critical role in the nucleation of microtubules. Western blotting and immunofluorescent confocal microscopy were used to study the assembly and transformation of γ-tubulin during meiotic maturation, fertilization and parthenogenetic activation of pig oocytes. γ-Tubulin exists in the porcine oocytes and its quantity remains stable during meiotic maturation. It is located in the region where there is microtubule assembly, especially on the spindle poles at metaphase and middle plate or midbody of elongating spindle at anaphase and telophase. After in vitro fertilization and parthenogenetic activation, γ-tubulin concentrated around the male and female chromatin and pronuclei. It is also found to be localized in sperm acrosomal cap and the neck. During early cleavage, γ-tubulin distributes around the blastomere nuclei. The results suggest that γ-tubulin is a major regulator of microtubule assembly in porcine eggs and that both oocyte and sperm contribute centrosome materials to fertilized eggs.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2004年第4期339-344,共6页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目 ( 3 0 2 2 5 0 10 ,3 0 170 3 5 8)
国家重点基础研究发展规划项目 ("973") (G19990 5 5 90 2 )
中国科学院创新工程项目 (KSCX3 IOZ 0 7)~~
关键词
Γ-微管蛋白
猪
卵母细胞
孤雌活化
精子
受精
γ-tubulin, oocyte, sperm, fertilization, parthengenetic activation
