摘要
目的 观察先天性巨结肠症 (HD)患者细胞黏附分子 (CAMs)在神经节正常肠段和神经节缺如肠段的表达情况。探讨CAM 成纤维细胞生长因子 (FGFR)信号传导在HD致病机制中的作用。方法 应用链酶抗生素蛋白 生物素 过氧化酶复合体法 (SABC) ,检测 16例HD患者中神经节正常和缺如肠段中CAMs的表达。结果 神经节细胞黏附分子 (NCAM )和神经元细胞钙黏素(N cadherin)在神经组织和平滑肌组织中都有表达 ,但在神经节正常肠段 (NG )肠组织中NCAM和N cadherin染色的神经纤维的数量明显多于神经节缺如肠段 (AG)。结论 CAMs在AG肠段的明显减少说明HD患者中CAM FGFR信号传导发生了异常 ,可能是导致肠神经母细胞移行障碍的原因之一。
Objective To investigate the expression of cell adhesion molecule (CAMs) in ganglionic (NG) and aganglionic (AG) segments of Hirschsprung disease (HD) in order to understand the role of CAM-FGFR signaling in the pathogenesis of HD.Methods The SABC immunohistochemical method was used in the experiment to detect the expression of CAMs both in 16 NG and AG segments of HD.Results NCAM and N-cadherin were detected both in nerve tissue and myenteric plexus,but numerous NCAM-positive and N-cadherin-positive fine fibres were present in NG segment,only a few were present in AG segment.Conclusion The markedly decreased expression of CAMs on nerve fibres within the muscle of AG segment suggests that CAM-FGFR signaling is altered in HD,resulting in failure of enteric neuroblast migration.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2004年第2期218-219,共2页
Chinese Journal of Experimental Surgery
