摘要
目的 探讨塞莱昔布对乳腺癌细胞系的凋亡诱导作用和对环氧化酶 2蛋白的表达抑制作用。方法 以雌激素受体ER( )的人乳腺癌细胞系MDA MB 2 3 1为研究对象 ,已知环氧化酶 2蛋白高表达的人胃癌细胞系SGC 790 1为阳性对照。分别经不同浓度塞莱昔布作用 2 4h后 ,Westernblot检测环氧化酶 2蛋白的表达 ;凋亡DNA琼脂糖凝胶电泳检测DNA凋亡梯度。ImageTool凝胶分析软件分析Westernblot电泳图像。结果 DNA梯度随塞莱昔布的浓度加大而加大。Westernblot结果示环氧化酶 2蛋白的表达随着塞莱昔布的浓度加大而明显抑制 ,在浓度为 10 0μmol/L时 ,无法检测到环氧化酶 2蛋白的表达。ImageTool软件分析Westernblot灰度值差异有显著性 (P <0 .0 1)。结论 塞莱昔布有良好的凋亡诱导作用 ,其凋亡诱导可能与环氧化酶 2蛋白的表达抑制有关。
Objective To study the effects of celecoxib in inducing apoptosis and suppressing the expression of cyclooxygenase-2 protein in human breast cancer cell lines MDA-MB-231.Methods The human breast cancer cell line MDA-MB-231 was studied and SGC-7901 served as the positive control cell line.Cells were cultured for 24 h with celecoxib at different concentrations.Apoptosis was detected by DNA fragmentation electrophoresis,while the COX-2 protein by Western blot.Image Tool software was used to analyze the Western blotting image.Results With the increase of celecoxib concentrations,cell apoptosis was increased.The COX-2 protein expression could be obviously suppressed by COX-2 specific inhibitor celecoxib.The concentration of maximum suppressing effect was 100 μmol/L.Softwareanalysis revealed that with the increase of celecoxib concentrations,the expression ofCOX-2 protein was obviously decreased with the difference being significant (P<0.01).Conclusion Celecoxib could effectively induce apoptosis in MDA-MB-231 cell line,which may be related to the expression inhibition of COX-2 protein.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2004年第2期158-159,共2页
Chinese Journal of Experimental Surgery
