摘要
目的 探讨不同孕龄孕鼠子宫、胎盘缺血对胎鼠体重及神经系统发育的影响。方法利用子宫动脉钳夹法对妊娠 13d(缺血 1组 )及妊娠 17d(缺血 2组 )孕鼠实施单侧子宫动脉缺血手术 ,制成孕鼠子宫动脉缺血模型。缺血组孕鼠的另一侧子宫动脉不钳夹为自身对照 ,相应为对照 1组和对照 2组。比较缺血组孕鼠子宫动脉钳夹缺血侧 ,与自身对照组孕鼠子宫动脉不钳夹侧 (无缺血侧 )的胎鼠体重、脑重及脑组织生长相关蛋白 4 3(GAP 4 3)mRNA的表达。结果 ( 1)缺血 1组胎鼠体重、脑重分别为 3 2 g和 0 16 g,显著低于对照 1组的 3 6g和 0 18g(P <0 0 1,P <0 0 1) ;缺血 2组的胎鼠体重、脑重分别为 3 1g和 0 16 g ,也显著低于对照 2组的 3 7g和 0 17g(P <0 0 1,P <0 0 5 )。 ( 2 )缺血 1组和对照 1组胎鼠脑组织GAP 4 3mRNA的表达分别为 1 10和 1 12 ,两组比较 ,差异无显著性(P >0 0 5 ) ;缺血 2组与对照 2组胎鼠脑组织GAP 4 3mRNA的表达分别为 1 0 6和 1 2 1,两组比较 ,差异有极显著性 (P <0 0 1)。结论 不同孕龄孕鼠子宫胎盘缺血均会显著抑制胎鼠的生长发育 ;妊娠晚期宫内缺血使胎脑组织GAP 4 3mRNA的表达显著减少 ,可对胎鼠的神经系统发育产生影响。
Objective To discuss the effects of utero placental ischemia on the body and nervous system development in fetal rats Methods By clamping the unilateral uterine artery of the rat, we produced a utero placental ischemia model The opposite uterus of the rat with normal uterine artery supply served as control We compared the body weight, weight of brain, and the expression of growth associated protein 43(GAP 43) mRNA in cerebral tissue by RT PCR in the 13 day (group 1) and 17 day(group 2) old fetal rats respectively Results The body weight and weight of brain in group 1 were 3 2 g and 0 16 g respectively, significantly lower than those of control 1 of 3 6 g and 0 18 g respectively ( P <0 01, P <0 05). The body weight and weight of brain in group 2 were 3 1 g and 0 16 g respectively, also significantly lower than those of control 2 of 3 72 g and 0 17 g respectively( P <0 01, P <0 05) The expression of GAP 43 mRNA in cerebral tissue of the group 1 (1 10) and its control(1 12) showed no obvious difference ( P >0 05) However, GAP 43 mRNA in cerebral tissue of the group 2 (1 06) was significantly decreased compared with that of its control(1 21 )( P <0 01) Conclusion Clamping the uterine artery can mimic intrauterine ischemia and produce FGR model Intrauterine ischemia in the late stage of pregnancy may decrease the expression of GAP 43 mRNA, which might affect the development of nervous system in rats
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2004年第4期221-223,共3页
Chinese Journal of Obstetrics and Gynecology
