摘要
目的 探讨生长激素对胰腺癌细胞株Bxpc 3CyclinD1表达与增殖活性的关系。 方法采用MTT法检测胰腺癌细胞株Bxpc 3增殖活性 ,Tunel法标记凋亡阳性细胞 ,激光共聚焦显微镜计数免疫荧光标记Tunel阳性细胞和增殖细胞 ,免疫组化检测CyclinD1的表达。 结果 10 -7、10 -8、10 -9mol/L浓度的生长激素在第 2天均明显的抑制胰腺癌细胞生长 ,伴随作用时间的延长这种抑制作用逐渐减轻 ,且出现了加速胰腺癌细胞生长的作用 ,第 4天、第 5天胰腺癌细胞的生长同正常对照组相比差异有显著意义 (P <0 0 1)。CyclinD1的表达同MTT的检测结果正相关 ,同凋亡结果负相关。结论 CyclinD1的检测可以直接反应生长激素对胰腺癌细胞的生长调节作用。
Objective To observe the growth effect of somatostapin on human pancreas cancer lines Bxpc-3. Methods The Bxpc-3 pancreas cancer cells were treated with Somatotropin. The cells hyperplasia were detected by MTT and were observed apoptosis cells determinated quantitatively by TUNEL, quantify immune fluoresence double marked the proliferation cells and apoptosis cells, the expression of Cyclin D1 detected by immunohistochemical. Results The growth effect of pancrea cancer cells were limited by 10 -7M?10 -8M?10 -9M Somatotropin on 2 day. The limited effect was decreased from 3 day. The cells proliferation were increased by somotostapin on 4day to 5day. The relationship between the expression of Cyclin D1 and apoptosis was negative correlation and the cells hyperplasia was positive correlation in Bxpc-3 cell line. Conclusion From the cell study we knew the expression of Cyclin D1 reflected the prolefiration of pancreas cancer cells.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2004年第7期432-434,共3页
Chinese Journal of Surgery
