摘要
目的 探讨过氧化物酶体增生物激活受体 γ激动剂———罗格列酮对单侧输尿管梗阻 (UUO)大鼠肾间质纤维化的影响。方法 将 30只Wistar大鼠随机分成罗格列酮治疗组、模型组和假手术组。对治疗组 ,模型组行单侧输尿管结扎术建立输尿管梗阻的动物模型。术后第 2 1天取 3组术侧肾组织做HE和Masson三色病理染色及用免疫组化方法检测α 平滑肌肌动蛋白 (α SMA)、转化生长因子 β1(TGF β1)和Ⅰ型胶原 (ColⅠ )在肾组织的表达水平。结果 罗格列酮可显著下调α SMA、TGF β1的表达 ,减轻Ⅰ型胶原在肾间质中的沉积 ,改善了肾脏病理改变。
Objective To study the effects of peroxisome protiferator activated receptor gamma (PPARγ) agonist rosiglitazone on the renal interstitial fibrosis caused by unilateral ureteral obstruction (UUO) in rats. Methods Thirty female Wistar rats were randomly subdivided into rosiglitazone-treated group, UUO model group and sham operated group. The rats in the rosiglitazone treated group and UUO model group underwent UUO. After 21 days of the operation, all animals were sacrificed and the resected kidneys were collected for HE and MASSON staining and immunohistochemical staining for α SMA, TGT β1 and collagen I.Results In the obstructed kidneys, there was significant renal interstieial fibrosis and interstitial space expansion. Immunohistochemical staining showed that the expression of α SMA, TGF β1 and Col I was significantly increased in the obstructed kidneys. In comparison with the model group, rosiglitazone could obviously decrease interstitial volume and the expression of α SMA, TGF β1 and Col I. It also improved the histological changes of the UUO renal tissue. Conclusion PPARγ agonist rosiglitazone can down regulate the expression of α SMA, TGF β1 and Col I in UUO rats. This demonstrated its potential ability to inhibit renal interstitial fibrosis caused by UUO in rats.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2004年第2期150-153,242,共5页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
