摘要
目的 研究非小细胞肺癌患者拓扑异构酶Ⅱα (TopoisomeraseⅡα ,TopoⅡα)的表达 ,并探讨其与Ki6 7蛋白、p5 3基因的关系。 方法 免疫组织化学SP法检测 94例无术前化疗史的非小细胞肺癌患者肿瘤组织TopoⅡα、Ki6 7蛋白的表达 ,并用聚合酶链反应 单链构象多态性 (PCR SSCP)分析p5 3基因 5、 6、 7、 8外显子的突变。结果TopoⅡα、Ki6 7蛋白阳性率分别为 30 % ( 2 8/ 94 )和 39% ( 37/ 94 ) ,且TopoⅡα的表达在鳞癌中明显高于腺癌 (P <0 0 5 ) ,中、低分化的肿瘤组织高于分化较好的肿瘤 (P <0 0 5、P <0 0 1) ,吸烟患者高于非吸烟患者 (P <0 0 1)。等级相关结果显示TopoⅡα的表达与Ki6 7呈正相关 (r =0 7381,P <0 0 1)且与p5 3基因也相关 (r =0 2 6 2 3,P<0 0 5 ) ,p5 3基因突变型组TopoⅡα的表达显著高于野生型组 (P <0 0 5 )。 结论 ①TopoⅡα的表达与肿瘤分化程度以及核增殖抗原Ki6 7显著相关 ,其检测可作为研究非小细胞肺癌增殖活性、分化程度的特异指标。②p5 3基因与TopoⅡα的相关性提示TopoⅡα的过表达可能受 p5
Objective To investigate the expression of topoisomerase Ⅱα (Topo Ⅱα) and its relationship with Ki67 protein, p53 gene in nonsmall cell lung carcinoma (NSCLC). Methods Surgically resected tumor specimens from 94 NSCLC patients who were not treated with preoperative chemotherapy were studied. Immunohistochemistry method (SP) was used to detect Topo Ⅱα, Ki67 protein, and polymerase chain reaction single strand conformation polymorphism was performed to investigate mutations of p53 gene. Results The expression rate of Topo Ⅱα and Ki67 was 30 % and 39 % respectively. Topo IIα expression in squamous cell carcinomas was significantly higher than in adenocarcinomas ( P <0 05). Topo Ⅱα expression in moderately differentiated tumors and poorly differentiated tumors was significantly higher than in well differentiated tumors ( P <0 05 and P <0 01, respectively). With respect to clinicopathologic parameters, TopoⅡα expression was also significantly higher in smokers than that in nonsmokers ( P <0 01). In addition, the expression of Topo Ⅱα was positively related with Ki67 (Spearman's r = 0 738 1 , P <0 01), and correlation was also found between Topo Ⅱα and p53 gene (Spearman's r =0 262 3 , P <0 05). Topo Ⅱα expression in tumors with mutant p53 was significantly higher than in those with wild-type p53 ( P <0.05). Conclusion Topo Ⅱα expression is significantly associated with the differentiation of tumors and Ki67 protein. Topo Ⅱα protein is an important indictor for predicting proliferative behavior of NSCLC, and correlation between Topo Ⅱα and p53 gene suggests that overexpression of Topo Ⅱα is induced by mutant p53.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2004年第2期118-121,F004,共5页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家青年基金资助项目 (No .0 4 835 10 0 35 )
