摘要
目的 探讨琥珀酸对人成纤维细胞损伤的机制及其在脆弱类杆菌感染中的作用。方法 分别以 5、10、2 0、30mmol L(pH 5.5 )的琥珀酸刺激体外培养的人成纤维细胞 ,2 4h后检测细胞活力、细胞凋亡率、培养上清中的胶原合成量及caspase 3的活性。以等渗盐水刺激的该细胞作为对照组 ,观察刺激前、后各指标的变化。 结果 随着琥珀酸浓度的增高 ,成纤维细胞增殖速度减慢 ,胶原合成量下降 ,细胞凋亡率及caspase 3活性上升。当琥珀酸浓度为 10 30mmol L时 ,caspase 3的活性为 (0 .5 70 7± 0 .0 345 )(0.5 90 4± 0.0 16 8)U,明显高于对照组 (P <0.0 5);当琥珀酸浓度为 2 0、30mmol L时 ,细胞活力及胶原合成量明显低于对照组 ,凋亡率则较之明显增高 (P <0.0 5)。 结论 琥珀酸能抑制成纤维细胞增殖及胶原合成 ,促进细胞凋亡。创面感染时 ,细菌可通过代谢产生琥珀酸抑制创面愈合 ,使感染持续存在。
Objective To explore the mechanism of injurious effect of succinic acid on human fibroblast and it′s role in bacteroides fragilis infection. Methods In vitro cultured human fibroblasts were challenged by succinic acid in concentrations of 5, 10, 20 and 30 mmol/L(pH5.5), respectively. The cellular activity, apoptosis rate, the collagen synthesis in the supernatant of the cell culture, and the activity of caspase-3 were determined 24 hours after challenge. Isotonic saline challenged fibroblast were employed as control and the changes in the indices before and after succinic acid challenge were observed. Results Along with the increase in the concentration of succinic acid, the fibroblast proliferation rate was decreased and so was the collagen synthesis. But the apoptosis rate and caspase-3 activity were increased. The activity of caspase-3 was markedly higher than that in normal control when the succinic acid concentration was 10～30 mmol/L. The cellular activity and collagen synthesis were significantly lower and the apoptosis rate was obviously higher than those in control group when the succinic acid concentration was 20 or 30 mmol/L(P<0.05). Conclusion The proliferation and collagen synthesis in fibroblast culture could be significantly inhibited and the cellular apoptosis could be promoted by succinic acid. The process of wound healing of the wounds infected by bacteroides fragilis would be delayed due to the production of succinic acid by the bacteria.
出处
《中华烧伤杂志》
CAS
CSCD
2004年第1期34-36,共3页
Chinese Journal of Burns
基金
国家自然科学基金资助项目 (3 9970 75 4)
