摘要
目的 探讨饮食中添加烟酸对染矽尘大鼠早期诱导型一氧化氮合酶 (iNOS)表达的影响。方法 将Wistar大鼠随机分为 3个实验组 ,即对照组、矽肺组和烟酸组 ,每组 4 8只。采用气管暴露法建立矽肺动物模型。高效液相色谱法测定血浆中烟酸含量。链菌素 过氧化物酶法结合组织芯片技术检测肺组织iNOS的表达。用Image ProPlus图像分析仪对iNOS的表达做定量分析。结果与对照组和矽肺组比较 ,染矽尘后 1、3、7、14、2 1、2 8d时 ,烟酸组大鼠血浆中烟酸浓度分别高出 5 .94 6 4、17.4 2 2 0、2 1.3980、16 .0 910、4 .4 14 3和 7.130 5mg/L ,差异均有显著性。与对照组比较 ,染矽尘后 7d ,肺组织iNOS积分吸光度值上升 2 7342 1,支气管肺泡灌洗上清液中一氧化氮合酶浓度 (NOS)和iNOS活力分别上升 2 6 1和 1 89kU/L ,差异均有显著性。烟酸治疗可使矽尘诱导的肺组织iNOS积分吸光度值降低 2 4 82 92 ,支气管肺泡灌洗上清液中NOS和iNOS活力的增加值分别降低 1 5 0和 0 91kU/L ,差异均有显著性。
Objective To evaluate the effects of niacin supplemented in diet on temporal expression of nitric oxide synthase in rat lung exposed to silica by tissue array technology Methods Wistar rats were randomly divided into three experimental groups: saline treated group, silica treated group, niacin treated group. There are 48 animals in each group. Animal models were established by direct tracheal instillation of silica into the rat lungs Plasma level of niacin was measured by high performance liquid chromatography (HPLC) The expression of iNOS protein in the paraffin embedded lung sections was measured with streptavidin/peroxidase(SP)immunohistochemistry on tissue microarray and quantified by Image Pro Plus Results Plasma level of niacin in niacin treated group were significantly elevated by 5.946 4,17.422 0, 21.398 0, 16.091 0, 4.414 3 and 7.130 5 mg/L at 1,3,7,14,21 and 28 days after instillation of silica, as compared to control and silica treated groups. Seven days after instillation of silica, iNOS integrated optical density (IOD) of the lung, total NOS and iNOS activities in bronchial alveolar lavage fluid (BALF) supernatant in silica treated group significantly elevated by 273 421, 2 61 kU/L and 1 89 kU/L, respectively, in the saline treated group, with statustucal significance Niacin treatment could significantly decrease silica elevated iNOS integrated optical density (IOD) of the lung, total NOS and iNOS activities in BALF supernatant by 248 292, 1 50 kU/L and 0 91 kU/L in the silica treated group, respectively, with statistical significance Conclusions It is suggested that treatment with niacin could effectively attenuate the over expression of nitric oxide synthase in the rat lung induced by silica particles in our study
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2004年第3期186-189,共4页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金资助项目 (3 0 0 70 65 4)
