摘要
概述了研究土壤微生物多样性的主要方法 .传统上 ,土壤微生物群落的分析依赖于培养技术 ,使用各种培养基最大限度地培养各种微生物群体 ,但仍只能培养和分离出一小部分土壤微生物群落 .使用Biolog分析、磷脂脂肪酸分析和核酸分析等方法 ,可研究和表征那些现在还不能够被培养的土壤微生物 ,从而获取关于土壤微生物群落多样性的更多和更完整的信息 .
This paper gave a review on the main methods for studying soil microbial diversity.Traditionally,the analysis of soil microbial communities relied on culturing techniques,using a variety of culture media.However,only a small fraction of the soil microbial community has been cultured and isolated with this approach.Other methods such as Biolog GN analysis,phospholipids fatty acids analysis and nucleic acid-based analysis can be used to study and characterize soil microbes which currently cannot be cultured,and to get more and complete information about soil microbial community.
出处
《应用生态学报》
CAS
CSCD
2004年第5期899-904,共6页
Chinese Journal of Applied Ecology
基金
国家杰出青年基金资助项目 ( 4 0 12 5 0 0 4)
