摘要
目的 :研究两种方法制备的不同壳聚糖多孔膜的理化性能和生物相容性 ,构建一种新型的生物因子盖髓载体。方法 :利用冷冻诱导相分离 (cryogenicinducedphaseseparation ,CIPS)的方法 ,分别在 - 5℃和 - 2 0℃制备两种不同的壳聚糖多孔膜 ,利用扫描电镜观察两者的微观形态特征 ,并对两者孔隙率和在 0 .0 1mol/L磷酸盐缓冲液 (PBS)中 30d的体外降解率进行比较。L92 9细胞与壳聚糖多孔膜复合培养 ,进行扫描电镜观察。结果 :两种膜均为不对称结构 ,一侧为多孔层 ,一侧为致密层。 - 5℃制备的膜多孔层的孔径在 5 0～ 10 0 μm之间 ,结构均匀 ,孔隙率 93.2 % ,降解率 2 5 .2 % ;- 2 0℃制备的膜多孔层的孔径在 10 0～ 4 0 0 μm之间 ,结构不均匀 ,孔隙率78.2 % (P <0 .0 1) ,降解率 19.5 % (P <0 .0 5 )。L92 9细胞可深入孔隙内部并以球形状态黏附于材料表面。结论 :- 5℃制备的壳聚糖多孔膜结构均匀、孔径适宜、孔隙率高 ,可均匀地吸附外源性生物因子 ,并能起到良好的细胞支架作用 ,更适合作为外源性生物因子载体应用于活髓保存的研究。
AIM:To study the physico-chemical properties and biocompatibility of porous chitosan membranes,a noval biological factor carrier in pulp capping, prepared by two kinds of method.METHODS:By the method of cryogenic induced phase separation(CIPS) two kinds of porous chitosan membrane were prepared at -5℃ and -20℃ respectively.Micromorphology of the membranes were examined under scanning electron microscope.Their porosities and degradation in 0.01 mol/L PBS solution for 30 days were compared.L929 cells were co-cultured with the porous chitosan membranes and morphological observation was performed with scanning electron microscope.RESULTS:Asymme- tric chitosan membranes including porous and dense layers were prepared by CIPS method.The size of poruses of membranes prepared at -5℃ was about 50～100 μm with regular structure. Porosity and degradation were 93.2% and 25.2% respectively;The size of poruses of membranes prepared at -20℃ was about 100～400 μm with irregular structure.Porosity and degradation were 78.2%( P <0.01)and 19.5%( P <0.05).L929 cells could grow into the poruses and attach the material with globular morphology.CONCLUSION:The membranes prepared at -5℃ with advantages of regular structure,high porosity and suitable degradation could distribute the biological factors regularly,and could act as a fine scaffold for cell growth.It was more suitable to be used as biological factor carriers for vital pulp preservation.
出处
《牙体牙髓牙周病学杂志》
CAS
2004年第4期207-210,共4页
Chinese Journal of Conservative Dentistry
关键词
壳聚糖
多孔
载体
活随保存
chitosan
porosity
carrier
vital pulp conservation.
