摘要
目的 :在真核细胞中表达人lrg基因。 方法 :构建野生型人lrg的真核表达载体 ,体外转染肝癌细胞系HepG2 ,并进行稳定筛选。用WesternBlot、SABC -FITC进行人lrg基因表达的鉴定。 结果 :成功构建了人lrg的真核表达载体 pcDNA3.1( + ) /hlrg ,WesternBlot和SABC -FITC等实验表明该基因在HepG2中进行了表达。结论 :在HepG2中稳定表达了 pcDNA3.1( + ) /hlrg ,为深入探讨人lrg基因的功能奠定了基础。
AIM:To expression human lrg in eukaryotic cells.METHODS:Eukaryotic expressive vector of human lrg was constructed, and then the plasmid was transfected into HepG2 cell line using lipid transfection reagent.Expression of lrg was detected by Western Blot and SABC-FITC.RESULTS:Construction of eukaryotic expression vector pcDNA3.1(+)/ hlrg ,and expression of hlrg in HepG2 were confirmed by Western Blot and SABC-FITC.CONCLUSION: Expression of pcDNA3.1(+)/ hlrg in HepG2 may throw light on the further study of the function of human lrg .
出处
《牙体牙髓牙周病学杂志》
CAS
2004年第4期179-182,共4页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金资助项目 (3 0 170 3 61)
