摘要
背景与目的 :建立一种稳定的人卵母细胞体外培养成熟技术。材料与方法 :取术后部分卵巢组织 ,穿刺卵泡获得未成熟卵母细胞 ,在含激素的培养基中培养24～36h。结果 :经培养的人卵母细胞合符成熟的判断标准 :①含有第一极体 ;②胞浆匀称 ,色浅 ,颗粒均匀。结论 :该方法稳定 。
BACKGROUND AIM: To establish a stable in vitro culture technique of human oocytes. MATERIAL AND METHODS: Immature human oocyte were achieved by puncture follicle and cultured for24to36hours in HTF Medium(containing hMG). RESULTS: Oocytes after cultured were judged as mature according to the two criteria:the first polar body existed;the cytoplasm was symmetric,light and gramule uniformly. CONCLUSION: The method was stable,this result suggested that the maturation in vitro of immature human oocyte could have great potential for human assisted reproduction.
出处
《癌变·畸变·突变》
CAS
CSCD
2004年第3期183-184,共2页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金(No.30170481)
关键词
卵母细胞
体外培养成熟
oocyte
maturation in vitro culture
