摘要
目的 比较弓形虫不同地理株 (RH株、ZS2株、GT株 ) GRA7基因的异同。方法 运用 PCR从弓形虫不同地理株的基因组 DNA中扩增出 GRA7基因 ,对 GRA7基因用限制性内切酶(Esp3I、Cfr I、Mbo I)酶切并比较。将 GRA7基因克隆至 p GEX- 4 T- 1质粒 ,转化大肠杆菌 JM10 9,进行测序并比较。结果 PCR扩增出 3株弓形虫的目的基因片段大小均在 5 0 0 bp- 75 0 bp之间 ,约711bp。 3种限制性内切酶酶切片段的大小均与理论值相符。 3株弓形虫 GRA7基因序列相同。结论 弓形虫不同地理株 GRA7基因具有高度保守性。
Objective To compare the difference of GRA7 genes from different geographical Toxoplasma gondii of RH strains,ZS 2 strains and GT strains.Methods The GRA7 gene was amplified by using polymerase chain reaction(PCR) technique from genomic DNA of different geographical Toxoplasma gondii strains. The GRA7 gene were digested by the restriction endonuclease (Esp3I,CfrI and MboI),and cloned into plasmid pGEX-4T-1.The reconstructed plasmid were transfered into E.coli JM109 and were sequenced and were comparied. Results The length of Gene fragements amplified from three Toxoplasma gondii.Strains was from 500 base pair to 750 base pair,averaged at 711 base pair.The length of three digested fragments by restriction endonuclease met with the theoretical length.Conclusion The GRA7 gene from different geographical Toxoplasma gondii strains (RH,ZS 2 and GT) has highly conservation.
出处
《新乡医学院学报》
CAS
2004年第3期173-176,共4页
Journal of Xinxiang Medical University
