摘要
AA肉鸡雏鸡分别经口免疫柔嫩艾美耳球虫毒株早熟株 ,用免疫组化ABC法染色检测小肠、盲肠扁桃体、脾脏中CD4 +、CD8+T淋巴细胞动态变化 ;用淋巴细胞转化实验MTT法检测T淋巴细胞活性。结果表明 :球虫免疫鸡后 ,CD4 +T淋巴细胞在一次免疫后迅速增殖 ,第 6天达到第一峰 ,免疫柔嫩艾美耳球虫毒株的鸡盲肠与脾脏中CD4 +T淋巴细胞占所有淋巴细胞百分率分别达到 38%和 4 4 % ,免疫早熟株的鸡盲肠与脾脏中CD4 +T淋巴细胞占所有淋巴细胞百分率分别达到 36 %和 4 3% ,而二次免疫后和对照相比很少有显著差异。CD8+T淋巴细胞在一次免疫后比CD4 +增殖速度慢 ,第 9天达到第一峰 ,免疫柔嫩艾美耳球虫毒株的鸡在此日盲肠与脾脏中CD8+T淋巴细胞占所有淋巴细胞百分率分别达到 37%和 4 7% ,免疫早熟株的鸡在此日盲肠与脾脏中CD8+T淋巴细胞占所有淋巴细胞百分率分别达到 36 %和 4 8% ,二次免疫后迅速大量增殖 ,免疫柔嫩艾美耳球虫毒株的鸡在二免后第2天盲肠与脾脏中CD8+T淋巴细胞占所有淋巴细胞百分率分别达到 39%和 5 0 % ,免疫柔嫩艾美耳球虫早熟株的鸡在二免后第 2天盲肠与脾脏中CD8+T淋巴细胞占所有淋巴细胞百分率分别达到 37%和 5 0 %。且CD8+T淋巴细胞总体水平比CD4 +T淋巴细胞多。
AA chickens were orally immunized with Eimeria tenella wild oocysts and its precocious oocysts twice. The oral infection and subsequent challenge with Eimeria oocysts effect on the populations of CD4 +?CD8 +T lymphocytes in the intestine, cecal tonsils and spleen of chickens, which was assessed by immunohistochemistry. Lymphocyte proliferation assay was used for the assessment of cellular responses. The results showed that after primary infection CD4 + cells increaseed immediately and significantly and reached peaked on 6th day, and the area positive of CD4 + cells of intestine and spleen was 38 % and 44 % in chickens immunized with wild oocysts, and was 36 % and 43 % in chickens immunized with precocious oocysts. Whereas nonspecific increase post challenge was found. The rapid and considerable increase of CD8+ cells occurred post challenge, on day 2nd post challenge, the area positive of CD8 + cells in intestine and spleen was 37 % and 50 % in chicken immunized with live oocysts, and was 39 % and 50 % in chickens immunized with precocious oocysts. No notable differences were observed between the immune responses against wild and precocious oocysts. The lymphocyte responses of immune chickens noticeably increased post infection than the control chicken. The response level of chickens post challenge was remarkabley higher than the level without challenge. There was not significant difference between response level of chickens immuneized with wild oocysts and chicken immune with precocious oocysts.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2004年第2期57-62,共6页
Journal of China Agricultural University
基金
国家自然科学基金资助项目 ( 30 170 6 95 )
