摘要
研究了聚吖啶红修饰玻碳电极的制备及肾上腺素在此修饰电极上的电化学行为。在pH7.4的磷酸盐缓冲溶液中,肾上腺素在修饰电极上呈现3个峰,一个还原峰和两个氧化峰,其峰电位随着pH的增加而负移。肾上腺素浓度在1.0×10-6～1.0×10-4mol L的范围内与其氧化峰电流呈线性关系,回归方程为ip(10μA)=1.160+0.4390c(mol L),相关系数r=0.9981,检出限为1.0×10-7mol L。实验结果表明:该修饰电极能有效消除抗坏血酸的干扰,方法用于注射液中肾上腺素的检测,其回收率在93.7%～100.3%范围内。
Poly (acridine red) modified glassy carbon electrode was prepared by electro-polymerization. Epinephrine was studied at the modified electrode by cyclic voltammetry and differential pulse voltammetry. Epinephrine had three peaks on the modified electrode, one cathodic peak and two anodic peaks. The peak potentials decreased with the increase of solution pH. The anodic peak current was in a linear relationship with epinephrine concentration in the range of 1.0×10^(-6)～1.0×10^(-4) mol/L. The linear regression equation was i_p(10 μA) =1.160+0.4390 c (mol/L), with a correlation coefficient of 0.9981. The detection limit (S/N=3) obtained by differential pulse voltammetry was 1.0×10^(-7) mol/L. The results showed that the modified electrode could diminish the interference of ascorbic acid. The proposed method was applied to the detection of epinephrine in samples. The recovery was in the range of 97.3%～100.3%.
出处
《分析试验室》
CAS
CSCD
北大核心
2004年第4期1-4,共4页
Chinese Journal of Analysis Laboratory
基金
安徽师范大学专项基金
安徽省骨干教师资助课题
