摘要
目的 通过观察糖尿病 (diabetesmellitus ,DM )大鼠视网膜神经节细胞 (retinalganglioncells ,RGCs)凋亡及对凋亡相关基因和神经营养因子表达的检测 ,探讨红参对RGCs的神经保护作用。方法 链脲佐菌素诱导实验性DM大鼠模型。治疗组每日红参粉末灌胃给药 1次。TUNEL法检测RGCs凋亡并记数RGCs凋亡数量。LSAB法检测bcl- 2、Bax、bFGF在RGCs层的表达 ,图象分析仪检测免疫组化的显色强度并进行定量分析。结果 DM大鼠RGCs凋亡数量显著增加 ,治疗组RGCs凋亡数量明显减少。LSAB法标记染色的bcl- 2、Bax、bFGF在DM大鼠视网膜RGCs层表达增强 ,bcl - 2、bFGF在治疗组表达增强 ,Bax表达减弱。bcl- 2、bFGF表达与RGCs凋亡数呈负相关 ,Bax表达与RGCs凋亡数呈正相关。结论 红参能促进bcl- 2和bFGF的表达 ,抑制bax的表达 ,减少DMRGCs的凋亡 。
OBJECTIVE The research was designed to assess the damage of the retinal ganglion cells(RGCs)in rats with DM and the genes related to apoptosis and the expression of the neurotrophic factors and discuss the neurological protection on RGCs by Ginseng METHODS Diabetic rats were induced by streptozotocin Rats were treated with Ginseng once each day in the treated group Apoptosis of RGCs was measured by TUNEL assay and apoptosis RGCs were counted LSAB method was used to measured by the expression level of bcl-2, Bax and bFGF in RGCs Immunological histochemistry assay was used for quantitative analysis RESULTS The number of apoptosis of RGCs were increased obviously in diabetes control group Expression level of bcl-2, Bax and bFGF was enhanced in the treated group Expression level of bcl-2 and bFGF was enhanced but Bax was decreased in treated group The expression level of bcl-2 and bFGF was correlated negatively with the number of apoptosis of RGCs while Bax was positively CONCLUSION Ginseng can induce the expression of bcl-2 and bFGF while inhibiting the Bax Ginseng can reduce the apoptosis of RGCs so that it could be proved to protect RGCs neurologically
出处
《中国中医眼科杂志》
2004年第2期63-66,共4页
China Journal of Chinese Ophthalmology
关键词
红参
视网膜神经节细胞
糖尿病
细胞凋亡
ginseng
retinal ganglion cells,RGCs
diabetes mellitus,DM
apoptosis
