摘要
目的 克隆人TALL 1基因全长及其胞外区片段 ,构建人TALL 1及其胞外区蛋白毕赤酵母分泌型表达载体。方法 从人新鲜淋巴结组织中提取总RNA ,利用RT PCR技术扩增人TALL 1及其胞外区基因编码区序列 ,构建人TALL 1及其胞外区cDNA毕赤酵母表达载体 ,并进行序列测定。结果 克隆获得的 85 8bp和 4 5 9bp片段与文献报道的人TALL 1全长及其胞外区基因编码区cDNA序列一致 ,将目的基因插入酵母表达载体pPIC 9Kα因子分泌信号肽下游。结论 本实验为大量获得人TALL 1蛋白及其可溶性功能蛋白 。
Objective To clone the human TALL-1 and the TALL-1 extracellular domain coding sequence and construct Pichia pastories expression vector of them. Methods Human TALL-1 cDNA and the TALL-1 extracellular domain cDNA was amplified with total RNA from human lymph node tissue by RT-PCR, then cloned into Pichia pastories expression vector pPIC-9K and sequenced. Results The cloned human TALL-1 cDNA and the TALL-1 extracellular domain cDNA sequence are identical to the published sequence and the gene's expression was under the alcohol oxidase(AOX1) promoter control and the coding sequence was fused to the alpha-mating factor signal. Conclusion These results slay foundations for further research in expression of TALL-1 and its clinical trial.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2004年第3期197-200,共4页
Immunological Journal
基金
重庆市院士基金资助项目 (7672 )
